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Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype

Cited 5 time in Web of Science Cited 6 time in Scopus
Authors
Yang, Jina; Kim, Beomhee; Kim, Gi Yeon; Jung, Gyoo Yeol; Seo, Sang Woo
Issue Date
2019-05-09
Publisher
BioMed Central
Citation
Biotechnology for Biofuels. 12(1):113
Keywords
Synthetic biologyEvolutionary engineeringBiosensorCell factoryDirected evolution
Abstract
With the increased attention on bio-based industry, demands for techniques that enable fast and effective strain improvement have been dramatically increased. Evolutionary engineering, which is less dependent on biological information, has been applied to strain improvement. Currently, synthetic biology has made great innovations in evolutionary engineering, particularly in the development of synthetic tools for phenotypic perturbation. Furthermore, discovering biological parts with regulatory roles and devising novel genetic circuits have promoted high-throughput screening and selection. In this review, we first briefly explain basics of synthetic biology tools for mutagenesis and screening of improved variants, and then describe how these strategies have been improved and applied to phenotypic engineering. Evolutionary engineering using advanced synthetic biology tools will enable further innovation in phenotypic engineering through the development of novel genetic parts and assembly into well-designed logic circuits that perform complex tasks.
ISSN
1754-6834
Language
English
URI
http://hdl.handle.net/10371/153914
DOI
https://doi.org/10.1186/s13068-019-1460-5
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Program in Bioengineering (협동과정-바이오엔지니어링전공)Journal Papers (저널논문_협동과정-바이오엔지니어링전공)
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