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Comparison of follicular fluid IGF-I, IGF-II, IGFBP-3, IGFBP-4 and PAPP-A concentrations and their ratios between GnRH agonist and GnRH antagonist protocols for controlled ovarian stimulation in IVF-embryo transfer patients

Cited 23 time in Web of Science Cited 27 time in Scopus
Authors
Choi, Young Sik; Ku, Seung-Yup; Jee, Byung-Chul; Suh, Chang-Suk; Choi, Young Min; Kim, Jung Gu; Moon, Shin Yong; Kim, Seok Hyun
Issue Date
2006-04-08
Publisher
Oxford University Press
Citation
Hum Reprod. 2006 Aug;21(8):2015-21. Epub 2006 Apr 6.
Keywords
AdultEmbryo TransferEnzyme-Linked Immunosorbent AssayEstradiol/bloodFemaleFertilization in VitroFollicular Fluid/*chemistryGonadotropin-Releasing Hormone/*agonists/analogs &derivatives/*antagonists & inhibitors/therapeutic useHumansInsulin-Like Growth Factor Binding Protein 3/*analysisInsulin-Like Growth Factor Binding Protein 4/*analysisInsulin-Like Growth Factor I/*analysisInsulin-Like Growth Factor II/*analysisOvulation Induction/*methodsPregnancyPregnancy RatePregnancy-Associated Plasma Protein-A/*analysisRadioimmunoassayTriptorelin/therapeutic use
Abstract
BACKGROUND: Insulin-like growth factors (IGF) and their binding proteins (IGFBP) play a major role in the autocrine and paracrine regulation of folliculogenesis. This is the first study that has compared follicular fluid (FF) IGF-I, IGF-II, IGFBP-3, IGFBP-4 and pregnancy-associated plasma protein (PAPP)-A concentrations, and their ratios, to investigate whether there was any difference in the intrafollicular microenvironment between the GnRH agonist (GnRHa) and antagonist (GnRHant) protocols for controlled ovarian stimulation (COS). METHODS: A total of 68 IVF cycles were included in this study; two groups were studied: GnRHa long protocol group (n = 36) and the flexible GnRHant multiple-dose protocol group (n = 32). FF was obtained from dominant follicles during oocyte retrieval and stored at -70 degrees C until assayed. IGF-I, IGF-II and IGFBP-3 concentrations were measured by radioimmunoassay and IGFBP-4 and PAPP-A by enzyme-linked immunosorbent assay. RESULTS: The duration of COS was significantly longer, and total dose of gonadotrophins used, serum estradiol (E(2)) levels on hCG day and the number of oocytes retrieved were significantly higher in the GnRHa long protocol group. The concentrations of FF IGF-II and IGFBP-4 were significantly higher, and the ratio of IGF-I/IGFBP-4 was significantly lower in the GnRHa long protocol group. Serum E(2) levels per mature follicle were not different between the two groups. CONCLUSIONS: Our data may indicate a difference of intrafollicular microenvironment between cycles using GnRHa long protocols and those using GnRHant protocols. However, the difference in microenvironment does not appear to result in a difference in clinical outcome.
ISSN
0268-1161 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16601008

http://hdl.handle.net/10371/15697
DOI
https://doi.org/10.1093/humrep/del091
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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