S-Space College of Medicine/School of Medicine (의과대학/대학원) Ophthalmology (안과학전공) Journal Papers (저널논문_안과학전공)
The role of clusterin in in vitro ischemia of human retinal endothelial cells
- Kim, Jeong Hun; Yu, Young Suk; Kim, Jin Hyoung; Kim, Kyu-Won; Min, Bon-Hong
- Issue Date
- Taylor & Francis
- Curr Eye Res. 2007 Jul-Aug;32(7-8):693-8.
- Apoptosis/drug effects; Blood-Retinal Barrier/physiology; Blotting, Western; Cell Hypoxia; Cell Survival; Cells, Cultured; Chromatography, Affinity; Clusterin/pharmacology/*physiology; Endothelium, Vascular/drug effects/*metabolism; Glucose/deficiency; Humans; Indoles/metabolism; Ischemia/*metabolism; Membrane Proteins/metabolism; Phosphoproteins/metabolism; Retinal Vessels/*metabolism; Staining and Labeling; von Willebrand Factor/metabolism
- Clusterin has been reported to be up-regulated in diverse pathophysiological stresses, but its role is controversial. In this study, we investigated the role of clusterin under in vitro ischemia of human retinal endothelial cells (HRECs). When HRECs were exposed to oxygen-glucose deprivation (OGD), clusterin expression increased, whereas von Willebrand factor (vWF), occludin, and zonula occludens (ZO-1) markedly decreased. Interestingly, loss of tight junction proteins and death of HRECs in OGD conditions were restored by clusterin treatment. Our results suggest that the enhanced clusterin in OGD conditions may play a protective role against ischemia-induced tight junction protein loss and HRECs death.
- 0271-3683 (Print)
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