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College of Medicine/School of Medicine (의과대학/대학원)
Surgery (외과학전공)
Journal Papers (저널논문_외과학전공)
A streamlined platform for high-content functional proteomics of primary human specimens
- Issue Date
- 2005-08-25
- Publisher
- Nature Publishing Group
- Citation
- Nat Methods. 2005 Sep;2(9):691-7
- Keywords
- Breast Neoplasms/diagnosis/*enzymology ; Equipment Design ; Equipment Failure Analysis ; Female ; Gene Expression Profiling/*methods ; Humans ; Neoplasm Proteins/analysis/*metabolism ; Proteome/analysis/*metabolism ; Proteomics/*instrumentation/methods ; Tumor Cells, Cultured ; Tumor Markers, Biological/analysis/*metabolism
- Abstract
- Achieving information content of satisfactory breadth and depth remains a formidable challenge for proteomics. This problem is particularly relevant to the study of primary human specimens, such as tumor biopsies, which are heterogeneous and of finite quantity. Here we present a functional proteomics strategy that unites the activity-based protein profiling and multidimensional protein identification technologies (ABPP-MudPIT) for the streamlined analysis of human samples. This convergent platform involves a rapid initial phase, in which enzyme activity signatures are generated for functional classification of samples, followed by in-depth analysis of representative members from each class. Using this two-tiered approach, we identified more than 50 enzyme activities in human breast tumors, nearly a third of which represent previously uncharacterized proteins. Comparison with cDNA microarrays revealed enzymes whose activity, but not mRNA expression, depicted tumor class, underscoring the power of ABPP-MudPIT for the discovery of new markers of human disease that may evade detection by other molecular profiling methods.
- ISSN
- 1548-7091 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16118640
https://hdl.handle.net/10371/15955
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- College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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