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Potential differentiation of human mesenchymal stem cell transplanted in rat corpus cavernosum toward endothelial or smooth muscle cells

Cited 0 time in webofscience Cited 72 time in scopus
Authors
Song, Y S; Lee, H J; Park, I H; Kim, W K; Ku, J H; Kim, S U
Issue Date
2007-04-27
Publisher
Nature Publishing Group
Citation
Int J Impot Res. 2007 Jul-Aug;19(4):378-85. Epub 2007 Apr 26.
Keywords
Adipocytes/physiologyAnimalsBone Marrow Cells/physiologyCell Differentiation/*physiologyCell LineEndothelial Cells/*physiologyFlow CytometryGenes, myc/physiologyHumansImmunohistochemistryMale*Mesenchymal Stem Cell TransplantationMicroscopy, FluorescenceMuscle, Smooth, Vascular/cytology/*physiologyPenis/*cytology/*physiologyPhenotypeRatsRats, Sprague-DawleyRetroviridae/geneticsSpine/cytology
Abstract
One of the causes of erectile dysfunction (ED) is the damaged penile cavernous smooth muscle cells (SMCs) and sinus endothelial cells (ECs). To investigate the feasibility of applying immortalized human mesenchymal stem cells (MSCs) to penile cavernous ECs or SMCs repair in the treatment of ED, the in vivo potential differentiation of the immortalized human MSCs toward penile cavernous endothelial or smooth muscle was investigated. One clone of immortalized human bone marrow mesenchymal stem cell line B10 cells via retroviral vector encoding v-myc were transplanted into the cavernosum of the Sprague-Dawley rats and harvested 2 weeks later. The expression of CD31, von Willebrand factor (vWF), smooth muscle cell actin (SMA), calponin and desmin was determined immunohistochemically in rat penile cavernosum. Multipotency of B10 to adipogenic, osteogenic or chondrogenic differentiation was found. Expression of EC specific markers (CD31 or vWF protein) and expression of SMC specific markers (calponin, SMA or desmin protein) were demonstrated in grafted B10 cells. When human MSCs were transplanted into the penile cavernosum, they have the potential to differentiate toward ECs or SMCs. Human MSCs may be a good candidate in the treatment of penile cavernosum injury.
ISSN
0955-9930 (Print)
Language
English
URI
http://www.nature.com/ijir/journal/v19/n4/abs/3901539a.html

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17460699

http://hdl.handle.net/10371/15957
DOI
https://doi.org/10.1038/sj.ijir.3901539
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College of Medicine/School of Medicine (의과대학/대학원)Urology (비뇨기과학전공)Journal Papers (저널논문_비뇨기과학전공)
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