Potential differentiation of human mesenchymal stem cell transplanted in rat corpus cavernosum toward endothelial or smooth muscle cells

Cited 0 time in webofscience Cited 72 time in scopus
Song, Y S; Lee, H J; Park, I H; Kim, W K; Ku, J H; Kim, S U
Issue Date
Nature Publishing Group
Int J Impot Res. 2007 Jul-Aug;19(4):378-85. Epub 2007 Apr 26.
Adipocytes/physiologyAnimalsBone Marrow Cells/physiologyCell Differentiation/*physiologyCell LineEndothelial Cells/*physiologyFlow CytometryGenes, myc/physiologyHumansImmunohistochemistryMale*Mesenchymal Stem Cell TransplantationMicroscopy, FluorescenceMuscle, Smooth, Vascular/cytology/*physiologyPenis/*cytology/*physiologyPhenotypeRatsRats, Sprague-DawleyRetroviridae/geneticsSpine/cytology
One of the causes of erectile dysfunction (ED) is the damaged penile cavernous smooth muscle cells (SMCs) and sinus endothelial cells (ECs). To investigate the feasibility of applying immortalized human mesenchymal stem cells (MSCs) to penile cavernous ECs or SMCs repair in the treatment of ED, the in vivo potential differentiation of the immortalized human MSCs toward penile cavernous endothelial or smooth muscle was investigated. One clone of immortalized human bone marrow mesenchymal stem cell line B10 cells via retroviral vector encoding v-myc were transplanted into the cavernosum of the Sprague-Dawley rats and harvested 2 weeks later. The expression of CD31, von Willebrand factor (vWF), smooth muscle cell actin (SMA), calponin and desmin was determined immunohistochemically in rat penile cavernosum. Multipotency of B10 to adipogenic, osteogenic or chondrogenic differentiation was found. Expression of EC specific markers (CD31 or vWF protein) and expression of SMC specific markers (calponin, SMA or desmin protein) were demonstrated in grafted B10 cells. When human MSCs were transplanted into the penile cavernosum, they have the potential to differentiate toward ECs or SMCs. Human MSCs may be a good candidate in the treatment of penile cavernosum injury.
0955-9930 (Print)
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Urology (비뇨기과학전공)Journal Papers (저널논문_비뇨기과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.