Publications
Detailed Information
Hypoxia-inducible factor-1 alpha inhibits self-renewal of mouse embryonic stem cells in Vitro via negative regulation of the leukemia inhibitory factor-STAT3 pathway
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Chul-Ho | - |
dc.contributor.author | Lee, Hyo-Jong | - |
dc.contributor.author | Cha, Jong-Ho | - |
dc.contributor.author | Kim, Jeong Hun | - |
dc.contributor.author | Kim, Kwang Rok | - |
dc.contributor.author | Kim, Ji-Hye | - |
dc.contributor.author | Yoon, Dae-Kwan | - |
dc.contributor.author | Kim, Kyu-Won | - |
dc.date.accessioned | 2009-11-26T06:12:28Z | - |
dc.date.available | 2009-11-26T06:12:28Z | - |
dc.date.issued | 2007-03-16 | - |
dc.identifier.citation | J Biol Chem. 2007 May 4;282(18):13672-9. Epub 2007 Mar 14. | en |
dc.identifier.issn | 0021-9258 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17360716 | - |
dc.identifier.uri | https://hdl.handle.net/10371/15960 | - |
dc.description.abstract | During mammalian embryogenesis, the early embryo grows in a relatively hypoxic environment due to a restricted supply of oxygen. The molecular mechanisms underlying modulation of self-renewal and differentiation of mouse embryonic stem cells (mESCs) under such hypoxic conditions remain to be established. Here, we show that hypoxia inhibits mESC self-renewal and induces early differentiation in vitro, even in the presence of leukemia inhibitory factor (LIF). These effects are mediated by down-regulation of the LIF-STAT3 signaling pathway. Under conditions of hypoxia, hypoxia-inducible factor-1alpha (HIF-1alpha) suppresses transcription of LIF-specific receptor (LIFR) by directly binding to the reverse hypoxia-responsive element located in the LIFR promoter. Ectopic expression and small interference RNA knockdown of HIF-1alpha verified the inhibitory effect on LIFR transcription. Our findings collectively suggest that hypoxia-induced in vitro differentiation of mESCs is triggered, at least in part, by the HIF-1alpha-mediated suppression of LIF-STAT3 signaling. | en |
dc.language.iso | en | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.subject | Animals | en |
dc.subject | Cell Differentiation/drug effects/*physiology | en |
dc.subject | Cell Hypoxia/physiology | en |
dc.subject | Cell Proliferation/drug effects | en |
dc.subject | Embryonic Development/physiology | en |
dc.subject | Embryonic Stem Cells/cytology/*metabolism | en |
dc.subject | Gene Expression Regulation, Developmental/drug effects/physiology | en |
dc.subject | Hypoxia-Inducible Factor 1, alpha | en |
dc.subject | Subunit/genetics/*metabolism/pharmacology | en |
dc.subject | Leukemia Inhibitory Factor/genetics/*metabolism | en |
dc.subject | Mice | en |
dc.subject | RNA, Small Interfering/genetics | en |
dc.subject | Receptors, OSM-LIF/genetics/metabolism | en |
dc.subject | Response Elements/physiology | en |
dc.subject | STAT3 Transcription Factor/genetics/*metabolism | en |
dc.subject | Signal Transduction/drug effects/*physiology | en |
dc.title | Hypoxia-inducible factor-1 alpha inhibits self-renewal of mouse embryonic stem cells in Vitro via negative regulation of the leukemia inhibitory factor-STAT3 pathway | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 정철호 | - |
dc.contributor.AlternativeAuthor | 이효종 | - |
dc.contributor.AlternativeAuthor | 차종호 | - |
dc.contributor.AlternativeAuthor | 김정훈 | - |
dc.contributor.AlternativeAuthor | 김광록 | - |
dc.contributor.AlternativeAuthor | 김지혜 | - |
dc.contributor.AlternativeAuthor | 윤대관 | - |
dc.contributor.AlternativeAuthor | 김규원 | - |
dc.identifier.doi | 10.1074/jbc.M700534200 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.