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Influence of strain and age differences on the yields of porcine islet isolation: extremely high islet yields from SPF CMS miniature pigs

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Authors
Kim, Jae Hyeon; Kim, Hyoung-Il; Lee, Kang-Woo; Yu, Jae Eun; Kim, Sun Hee; Park, Ho Sun; Park, Chung-Gyu; Ihm, Sung-Hee; Ha, Jongwon; Kim, Sang Joon; Lee, Hong Kyu; Ahn, Curie; Park, Kyong Soo
Issue Date
2007-01-12
Publisher
Blackwell Publishing
Citation
Xenotransplantation. 2007 Jan;14(1):60-6
Keywords
Aging/*physiologyAnimalsAnimals, Inbred StrainsCell Separation/*methodsFemaleIslets of Langerhans/*cytology/*surgery*Islets of Langerhans TransplantationMaleOrgan SizeSpecific Pathogen-Free OrganismsSwineSwine, Miniature/*classification/*physiology
Abstract
BACKGROUND: Porcine pancreas is a potential source of material for islet xenotransplantation. However, the difficulty in isolating islets, because of their fragility and the variability of isolation outcome in donor age and breed, represents a major obstacle to porcine islet xenotransplantation. In this study, we compared the islet isolation yield of specific pathogen-free (SPF) Chicago Medical School (CMS) miniature pigs with that of another miniature pig breed and market pigs from a local slaughterhouse. METHODS: Nine adult CMS miniature (ACM) pigs (>12 months), six young CMS miniature (YCM) pigs (6-7 months), four adult Prestige World Genetics (PWG) miniature (APM) pigs (>12 months), and 13 adult market (AM) pigs from a local slaughterhouse were used for islet isolation. RESULTS: The islet yield per gram of pancreas from ACM pigs (9589 +/- 2823 IEQ/g) was significantly higher than that from APM pigs (1752 +/- 874 IEQ/g, P < 0.05), AM pigs (1931 +/- 947 IEQ/g, P < 0.05), or YCM pigs (3460 +/- 1985 IEQ/g, P < 0.05). Isolated islets from ACM pigs were significantly larger than those from AM pigs or YCM pigs. The in vitro and in vivo function of isolated islets showed no difference among experimental groups. The pancreases of ACM pigs contained higher mean islet volume density percentages and larger size of islets than those of AM or APM pigs. CONCLUSIONS: We isolated extremely high yields of well-functioning islets from ACM pigs bred under SPF conditions. SPF CMS miniature pigs should be one of the best porcine islet donors for clinical porcine islet xenotransplantation.
ISSN
0908-665X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17214705

http://hdl.handle.net/10371/15974
DOI
https://doi.org/10.1111/j.1399-3089.2006.00364.x
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College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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