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Influence of strain and age differences on the yields of porcine islet isolation: extremely high islet yields from SPF CMS miniature pigs
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jae Hyeon | - |
dc.contributor.author | Kim, Hyoung-Il | - |
dc.contributor.author | Lee, Kang-Woo | - |
dc.contributor.author | Yu, Jae Eun | - |
dc.contributor.author | Kim, Sun Hee | - |
dc.contributor.author | Park, Ho Sun | - |
dc.contributor.author | Park, Chung-Gyu | - |
dc.contributor.author | Ihm, Sung-Hee | - |
dc.contributor.author | Ha, Jongwon | - |
dc.contributor.author | Kim, Sang Joon | - |
dc.contributor.author | Lee, Hong Kyu | - |
dc.contributor.author | Ahn, Curie | - |
dc.contributor.author | Park, Kyong Soo | - |
dc.date.accessioned | 2009-11-26T06:19:49Z | - |
dc.date.available | 2009-11-26T06:19:49Z | - |
dc.date.issued | 2007-01-12 | - |
dc.identifier.citation | Xenotransplantation. 2007 Jan;14(1):60-6 | en |
dc.identifier.issn | 0908-665X (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17214705 | - |
dc.identifier.uri | https://hdl.handle.net/10371/15974 | - |
dc.description.abstract | BACKGROUND: Porcine pancreas is a potential source of material for islet xenotransplantation. However, the difficulty in isolating islets, because of their fragility and the variability of isolation outcome in donor age and breed, represents a major obstacle to porcine islet xenotransplantation. In this study, we compared the islet isolation yield of specific pathogen-free (SPF) Chicago Medical School (CMS) miniature pigs with that of another miniature pig breed and market pigs from a local slaughterhouse. METHODS: Nine adult CMS miniature (ACM) pigs (>12 months), six young CMS miniature (YCM) pigs (6-7 months), four adult Prestige World Genetics (PWG) miniature (APM) pigs (>12 months), and 13 adult market (AM) pigs from a local slaughterhouse were used for islet isolation. RESULTS: The islet yield per gram of pancreas from ACM pigs (9589 +/- 2823 IEQ/g) was significantly higher than that from APM pigs (1752 +/- 874 IEQ/g, P < 0.05), AM pigs (1931 +/- 947 IEQ/g, P < 0.05), or YCM pigs (3460 +/- 1985 IEQ/g, P < 0.05). Isolated islets from ACM pigs were significantly larger than those from AM pigs or YCM pigs. The in vitro and in vivo function of isolated islets showed no difference among experimental groups. The pancreases of ACM pigs contained higher mean islet volume density percentages and larger size of islets than those of AM or APM pigs. CONCLUSIONS: We isolated extremely high yields of well-functioning islets from ACM pigs bred under SPF conditions. SPF CMS miniature pigs should be one of the best porcine islet donors for clinical porcine islet xenotransplantation. | en |
dc.language.iso | en | en |
dc.publisher | Blackwell Publishing | en |
dc.subject | Aging/*physiology | en |
dc.subject | Animals | en |
dc.subject | Animals, Inbred Strains | en |
dc.subject | Cell Separation/*methods | en |
dc.subject | Female | en |
dc.subject | Islets of Langerhans/*cytology/*surgery | en |
dc.subject | Male | en |
dc.subject | Organ Size | en |
dc.subject | Specific Pathogen-Free Organisms | en |
dc.subject | Swine | en |
dc.subject | Swine, Miniature/*classification/*physiology | en |
dc.subject | Islets of Langerhans Transplantation | - |
dc.title | Influence of strain and age differences on the yields of porcine islet isolation: extremely high islet yields from SPF CMS miniature pigs | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김재현 | - |
dc.contributor.AlternativeAuthor | 김형일 | - |
dc.contributor.AlternativeAuthor | 이강우 | - |
dc.contributor.AlternativeAuthor | 유재은 | - |
dc.contributor.AlternativeAuthor | 김선희 | - |
dc.contributor.AlternativeAuthor | 박호선 | - |
dc.contributor.AlternativeAuthor | 박정규 | - |
dc.contributor.AlternativeAuthor | 임성희 | - |
dc.contributor.AlternativeAuthor | 하종원 | - |
dc.contributor.AlternativeAuthor | 김상준 | - |
dc.contributor.AlternativeAuthor | 이홍규 | - |
dc.contributor.AlternativeAuthor | 안규리 | - |
dc.contributor.AlternativeAuthor | 박경수 | - |
dc.identifier.doi | 10.1111/j.1399-3089.2006.00364.x | - |
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