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Sulfur-containing amino acids block stretch-dependent K+ channels and nitrergic responses in the murine colon

Cited 42 time in Web of Science Cited 43 time in Scopus
Authors
Park, Kyu Joo; Baker, Salah A; Cho, Sang Yun; Sanders, Kenton M; Koh, Sang Don
Issue Date
2005-02-09
Publisher
Nature Publishing Group
Citation
Br J Pharmacol. 2005 Apr;144(8):1126-37
Keywords
Amino Acids/*pharmacologyAnimalsColon/*drug effects/physiologyFemaleMaleMiceMice, Inbred BALB CNitrergic Neurons/*drug effects/physiologyPotassium Channel Blockers/*pharmacologyPotassium Channels/*physiologySulfur Compounds/*pharmacology
Abstract
1. Efforts to determine the role of stretch-dependent K(+) (SDK) channels in enteric inhibitory neural responses in gastrointestinal muscles are difficult due to a lack of blocking drugs for SDK channels. 2. SDK channels are blocked by sulfur-containing amino acids. These compounds reduced the open probability of SDK channels in on and off-cell patches of murine colonic myocytes. L-Methionine was the most selective and had little or no effect on other known K(+) conductances in colonic myocytes. 3. Application of L-cysteine, L-methionine or DL-homocysteine depolarized intact muscles and enhanced spontaneous contractions. D-Stereoisomers of these amino acids were less effective than L-stereoisomers. 4. Pretreatment of muscles with tetrodotoxin, N(W)-nitro-L-arginine or 1H-[1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one reduced the depolarization responses to these compounds, suggesting that spontaneous neural activity and release of NO tonically activates SDK channels. 5. Nitrergic responses to nerve stimulation were reduced by sulfur-containing amino acids. 6. These data suggest that nitrergic inhibitory junction potentials are mediated, in part, by activation of SDK channels in murine colonic muscles.
ISSN
0007-1188 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15700022

http://hdl.handle.net/10371/15980
DOI
https://doi.org/10.1038/sj.bjp.0706154
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College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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