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Therapeutic use of granulocyte-colony stimulating factor could conceal residual malignant cells in patients with AML1/ETO+ acute myelogenous leukemia

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Authors
Moon, H W; Shin, S; Kim, H Y; Kim, Y R; Cho, H I; Yoon, S S; Park, S; Kim, B K; Chun, H; Kim, H C; Park, C J; Min, Y H; Lee, D S
Issue Date
2006-06-23
Publisher
Nature Publishing Group
Citation
Leukemia. 2006 Aug;20(8):1408-13. Epub 2006 Jun 22.
Keywords
AdolescentAdultChildCore Binding Factor Alpha 2 Subunit/*geneticsFemaleFlow Cytometry*Gene RearrangementGranulocyte Colony-Stimulating Factor/*therapeutic useHumansLeukemia, Myeloid, Acute/*drug therapy/*geneticsMaleMiddle AgedNeoplasm, ResidualPolymerase Chain ReactionReceptors, Granulocyte Colony-Stimulating Factor/analysis/genetics
Abstract
We have experienced a number of cases of AML1/ETO+ acute myelogenous leukemia that showed remission based on bone marrow (BM) morphological criteria, but that revealed clonal abnormalities in most cells by fluorescence in situ hybridization (FISH). Interestingly, most of these cases had AML with AML1/ETO rearrangement. The malignant cells were differentiated and considered mature cells after granulocyte-colony stimulating factor (G-CSF) treatment. To clarify the possible mechanisms underlying this phenomenon, we investigated the expression levels of G-CSFR in AML cells with AML1/ETO rearrangement by flow cytometry and real-time polymerase chain reaction (PCR). The number of AML1/ETO+ cells expressing G-CSFR at baseline was significantly higher than that of AML1/ETO- AML cells (2673 vs 522). In addition, the G-CSFR gene was more highly expressed in AML1/ETO+ cells than in AML1/ETO- cells by real-time PCR. This study reveals that cases showing remission after treatment with G-CSF mostly had leukemia with AML1/ETO rearrangement. This finding might be explained by the higher expression of G-CSF receptor in AML1/ETO+ cells than in AML1/ETO- cells. We recommend that remission should be confirmed by FISH, because malignant clones can be differentiated and masked in morphological examination or chromosome test, especially for AML with AML1/ETO rearrangement.
ISSN
0887-6924 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16791271

http://hdl.handle.net/10371/15984
DOI
https://doi.org/10.1038/sj.leu.2404286
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College of Medicine/School of Medicine (의과대학/대학원)Biomedical Engineering (의공학전공)Journal Papers (저널논문_의공학전공)
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