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Nitric oxide upregulates the cyclooxygenase-2 expression through the cAMP-response element in its promoter in several cancer cell lines
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Seok-Woo | - |
dc.contributor.author | Sung, Myung-Whun | - |
dc.contributor.author | Heo, Dae-Seog | - |
dc.contributor.author | Inoue, Hiroyasu | - |
dc.contributor.author | Shim, Seon-Hui | - |
dc.contributor.author | Kim, Kwang-Hyun | - |
dc.date.accessioned | 2009-11-26T06:36:23Z | - |
dc.date.available | 2009-11-26T06:36:23Z | - |
dc.date.issued | 2005-07-12 | - |
dc.identifier.citation | Oncogene. 2005 Oct 6;24(44):6689-98. | en |
dc.identifier.issn | 0950-9232 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16007171 | - |
dc.identifier.uri | https://hdl.handle.net/10371/15998 | - |
dc.description.abstract | We previously showed that nitric oxide (NO) induces overexpression of cyclooxygenase-2 (COX-2) and production of prostaglandin E(2) in cancer cells. Here, we investigated the mechanisms by which NO induces COX-2 expression in cancer cells. We found that the cAMP-response element (CRE) is a critical factor in NO-induced COX-2 expression in all cells tested. We found that in cancer cells, three transcription factors (TFs) - cAMP response element-binding protein (CREB), activating transcription factor-2 (ATF-2) and c-jun, bound the CRE in the COX-2 promoter, and their activities were increased by addition of the NO donor, S-nitroso-N-acetyl-D,L-penicillamine (SNAP). NO-induced activation of soluble guanylate cyclase (sGC), p38 and c-Jun NH(2)-terminal kinase (JNK) upregulated the three TFs, leading to COX-2 overexpression. Addition of dibutyryl-cGMP (db-cGMP) induced COX-2 expression in a manner similar to SNAP; this induction was blocked by a p38 inhibitor (SB202190), but not by a JNK inhibitor (SP600125). NO-induced cGMP was found to activate CREB and ATF-2 in a p38, but not c-jun-dependent manner, while NO induced JNK in a cGMP-independent manner, leading to subsequent activation of c-jun and ATF-2. These results suggest that the low concentrations of endogenous NO present in cancer cell may induce the expression of many genes, including COX-2, which promotes the growth and survival of tumor cells. | en |
dc.language.iso | en | en |
dc.publisher | Nature Publishing Group | en |
dc.subject | Activating Transcription Factor 2 | en |
dc.subject | Base Sequence | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Cyclic AMP Response Element-Binding Protein/physiology | en |
dc.subject | Cyclic GMP/physiology | en |
dc.subject | Cyclooxygenase 2 | en |
dc.subject | DNA Primers | en |
dc.subject | Enzyme Activation | en |
dc.subject | Enzyme Inhibitors/pharmacology | en |
dc.subject | Humans | en |
dc.subject | JNK Mitogen-Activated Protein Kinases/metabolism | en |
dc.subject | Membrane Proteins | en |
dc.subject | Nitric Oxide/*physiology | en |
dc.subject | Nitric Oxide Synthase/antagonists & inhibitors | en |
dc.subject | Prostaglandin-Endoperoxide Synthases/*genetics | en |
dc.subject | Proto-Oncogene Proteins c-jun/drug effects/physiology | en |
dc.subject | Transcription Factors/drug effects/*physiology | en |
dc.subject | Up-Regulation/*physiology | en |
dc.subject | p38 Mitogen-Activated Protein Kinases/metabolism | en |
dc.subject | Promoter Regions, Genetic | - |
dc.title | Nitric oxide upregulates the cyclooxygenase-2 expression through the cAMP-response element in its promoter in several cancer cell lines | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박석우 | - |
dc.contributor.AlternativeAuthor | 성명훈 | - |
dc.contributor.AlternativeAuthor | 허대석 | - |
dc.contributor.AlternativeAuthor | 심선휘 | - |
dc.contributor.AlternativeAuthor | 김광현 | - |
dc.identifier.doi | 10.1038/sj.onc.1208816 | - |
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