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PLANT U-BOX PROTEIN 10 negatively regulates abscisic acid response in Arabidopsis

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Authors
Seo, Jun Sung; Zhao, Pingzhi; Jung, Choonkyun; Chua, Nam-Hai
Issue Date
2019-07-27
Publisher
SpringerOpen
Citation
Applied Biological Chemistry. 62(1):39
Keywords
Abscisic acidANAC019ANAC055ArabidopsisMYC2PUB10
Abstract
MYC2 is well known as a positive regulator for abscisic acid (ABA) signaling but whether PLANT U-BOX PROTEIN 10 (PUB10) is involved in ABA responses has not been reported. Here, we show that the E3 ubiquitin ligase PUB10 modulates ABA signaling in Arabidopsis. PUB10ox (35S:PUB10-myc) and myc2 loss-of-function mutants were hyposensitive to ABA during germination, whereas pub10 loss-of-function and MYC2ox (35S:MYC2-GFP) mutants were hypersensitive. In addition, pub10 mutants showed hypersensitivity to high salt and osmotic stress during germination; by contrast, PUB10ox line displayed the opposite phenotype. ABA-induced expression of KIN2 (Cold- and ABA-Inducible Protein), RD22 (Responsive to Dehydration 22), ANAC019 (NAC Domain-Containing Protein 19), and ANAC055 (NAC Domain-Containing Protein 55) was enhanced in both pub10 and MYC2ox plants. Taken together, pub10 plants phenocopied MYC2ox plants, whereas PUB10ox plants phenocopied myc2 in ABA response. Our results provide evidence that PUB10 negatively regulates ABA signaling in Arabidopsis.
ISSN
2468-0842
Language
English
URI
http://hdl.handle.net/10371/160723
DOI
https://doi.org/10.1186/s13765-019-0446-0
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Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Journal Papers (저널논문_국제농업기술학과)
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