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Comparative genomics of Mycoplasma pneumoniae isolated from children with pneumonia: South Korea, 2010–2016
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Joon Kee | - |
dc.contributor.author | Seong, Moon-Woo | - |
dc.contributor.author | Shin, Dongjin | - |
dc.contributor.author | Kim, Jong-Il | - |
dc.contributor.author | Han, Mi Seon | - |
dc.contributor.author | Yeon, Youbin | - |
dc.contributor.author | Cho, Sung Im | - |
dc.contributor.author | Park, Sung Sup | - |
dc.contributor.author | Choi, Eun Hwa | - |
dc.date.accessioned | 2020-03-23T05:32:09Z | - |
dc.date.available | 2020-03-23T14:33:40Z | - |
dc.date.issued | 2019-11-29 | - |
dc.identifier.citation | BMC Genomics, 20(1):910 | ko_KR |
dc.identifier.issn | 1471-2164 | - |
dc.identifier.uri | 10.1186/s12864-019-6306-9 | - |
dc.identifier.uri | https://hdl.handle.net/10371/164757 | - |
dc.description.abstract | Background
Mycoplasma pneumoniae is a common cause of respiratory tract infections in children and adults. This study applied high-throughput whole genome sequencing (WGS) technologies to analyze the genomes of 30 M. pneumoniae strains isolated from children with pneumonia in South Korea during the two epidemics from 2010 to 2016 in comparison with a global collection of 48 M. pneumoniae strains which includes seven countries ranging from 1944 to 2017. Results The 30 Korean strains had approximately 40% GC content and ranged from 815,686 to 818,669 base pairs, coding for a total of 809 to 828 genes. Overall, BRIG revealed 99% to > 99% similarity among strains. The genomic similarity dropped to approximately 95% in the P1 type 2 strains when aligned to the reference M129 genome, which corresponded to the region of the p1 gene. MAUVE detected four subtype-specific insertions (three in P1 type 1 and one in P1 type 2), of which were all hypothetical proteins except one tRNA insertion in all P1 type 1 strains. The phylogenetic associations of 30 strains were generally consistent with the multilocus sequence typing results. The phylogenetic tree constructed with 78 genomes including 30 genomes from Korea formed two clusters and further divided into two sub-clusters. eBURST analysis revealed two clonal complexes according to P1 typing results showing higher diversity among P1 type 2 strains. Conclusions The comparative whole genome approach was able to define high genetic identity, unique structural diversity, and phylogenetic associations among the 78 M. pneumoniae strains isolated worldwide. | ko_KR |
dc.description.sponsorship | This research was supported by the 2017 Seoul National University Hospital Research Fund (0320170230) and the Basic Science Research Program through the National Research Foundation of Korea, which is funded by the Ministry of Education, Science and Technology (NRF2018R1D1A1A09082098). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. The study sponsors provided research grants to perform the study which was written by Dr. EH Choi. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Mycoplasma pneumoniae | - |
dc.subject | Whole genome analysis | - |
dc.subject | Comparative genomics | - |
dc.title | Comparative genomics of Mycoplasma pneumoniae isolated from children with pneumonia: South Korea, 2010–2016 | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이정기 | - |
dc.contributor.AlternativeAuthor | 성문우 | - |
dc.contributor.AlternativeAuthor | 신동진 | - |
dc.contributor.AlternativeAuthor | 김종일 | - |
dc.contributor.AlternativeAuthor | 한미선 | - |
dc.contributor.AlternativeAuthor | 연유빈 | - |
dc.contributor.AlternativeAuthor | 조성임 | - |
dc.contributor.AlternativeAuthor | 박성섭 | - |
dc.contributor.AlternativeAuthor | 최은화 | - |
dc.citation.journaltitle | BMC Genomics | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s). | - |
dc.date.updated | 2019-12-01T04:29:32Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 910 | ko_KR |
dc.citation.volume | 20 | ko_KR |
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