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An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development

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Authors
Lee, Sangyool; Joung, Young Hee; Kim, Ju-Kon; Choi, Yang Do; Jang, Geupil
Issue Date
2019-11-27
Publisher
BMC
Citation
BMC Plant Biology, 19(1):524
Keywords
ChloroplastPlastid-encoded RNA polymerasePEP-associated proteinFSD3, FSD3SAlternative splicing
Abstract
Background
Plastid-encoded RNA polymerase (PEP) plays an essential role in chloroplast development by governing the expression of genes involved in photosynthesis. At least 12 PEP-associated proteins (PAPs), including FSD3/PAP4, regulate PEP activity and chloroplast development by modulating formation of the PEP complex.

Results
In this study, we identified FSD3S, a splicing variant of FSD3; the FSD3 and FSD3S transcripts encode proteins with identical N-termini, but different C-termini. Characterization of FSD3 and FSD3S proteins showed that the C-terminal region of FSD3S contains a transmembrane domain, which promotes FSD3S localization to the chloroplast membrane but not to nucleoids, in contrast to FSD3, which localizes to the chloroplast nucleoid. We also found that overexpression of FSD3S negatively affects photosynthetic activity and chloroplast development by reducing expression of genes involved in photosynthesis. In addition, FSD3S failed to complement the chloroplast developmental defects in the fsd3 mutant.

Conclusion
These results suggest FSD3 and FSD3S, with their distinct localization patterns, have different functions in chloroplast development, and FSD3S negatively regulates expression of PEP-dependent chloroplast genes, and development of chloroplasts.
ISSN
1471-2229
Language
English
URI
https://doi.org/10.1186/s12870-019-2128-9

http://hdl.handle.net/10371/164760
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Journal Papers (저널논문_농생명공학부)
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