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Systematic analysis of expression signatures of neuronal subpopulations in the VTA

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Authors
Kim, Hyun Jin; Kim, Minhyung; Kang, Byeongsoo; Yun, Soyeon; Ryeo, Shin Eun; Hwang, Daehee; Kim, Joung-Hun
Issue Date
2019-12-11
Publisher
BMC
Citation
Molecular Brain, 12(1):110
Keywords
Allen brain atlasGene expression profileVTACell type markers
Abstract
Gene expression profiling across various brain areas at the single-cell resolution enables the identification of molecular markers of neuronal subpopulations and comprehensive characterization of their functional roles. Despite the scientific importance and experimental versatility, systematic methods to analyze such data have not been established yet. To this end, we developed a statistical approach based on in situ hybridization data in the Allen Brain Atlas and thereby identified specific genes for each type of neuron in the ventral tegmental area (VTA). This approach also allowed us to demarcate subregions within the VTA comprising specific neuronal subpopulations. We further identified WW domain-containing oxidoreductase as a molecular marker of a population of VTA neurons that co-express tyrosine hydroxylase and vesicular glutamate transporter 2, and confirmed their region-specific distribution by immunohistochemistry. The results demonstrate the utility of our analytical approach for uncovering expression signatures representing specific cell types and neuronal subpopulations enriched in a given brain area.
ISSN
1756-6606
Language
English
URI
https://doi.org/10.1186/s13041-019-0530-8

http://hdl.handle.net/10371/164794
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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