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Evaluation of factors related to Anaesthesia-induced Lens opacity in experimental mice

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dc.contributor.authorLee, Hun-
dc.contributor.authorKim, Hong Kyung-
dc.contributor.authorShin, Hae Sol-
dc.contributor.authorHan, Soo Jung-
dc.contributor.authorYoon, Sangchul-
dc.contributor.authorSeong, Je Kyung-
dc.contributor.authorSeo, Kyoung Yul-
dc.date.accessioned2020-04-03T06:33:48Z-
dc.date.available2020-04-03T15:34:36Z-
dc.date.issued2020-01-07-
dc.identifier.citationLaboratory Animal Research, 36(1):1ko_KR
dc.identifier.issn2233-7660-
dc.identifier.urihttps://doi.org/10.1186/s42826-019-0031-z-
dc.identifier.urihttps://hdl.handle.net/10371/164918-
dc.description.abstractTo investigate conditions that cause temporal lens opacity, we tested chemical and physical factors, such as anaesthesia dose, ocular surface dryness, and infrared (IR) light exposure in anaesthetised C57BL/6 N mice. Mice were anaesthetised with a low (80%; tiletamine/zolazepam 32 mg/kg and xylazine 8 mg/kg, intraperitoneal injection) or high (120%; 48 mg/kg and 12 mg/kg) dose of anaesthetic and examined every 5 min from 10 to 30 min after anaesthesia was induced. Lens opacity levels were assessed and graded (1–6) using the standard classification system. Regardless of the anaesthetic dose, lens opacity grade was 1–2 in moisturised eyes with application of 0.5% carboxymethylcellulose, and 5–6 in dry ocular surface conditions. Lens opacity in mice with high-dose anaesthetic in the dry ocular surface condition was not different from that of mice with low-dose anaesthetic. Lens opacity grade 1–2 was noted in eyes in the wet ocular surface condition, regardless of IR light exposure. During IR light exposure in eyes in the dry ocular surface condition, lens opacity (grade 6) in mice with high-dose anaesthetic was not different from that (grade 6) in mice with low-dose anaesthetic. We demonstrated that ocular surface dryness might be a relevant factor for the formation and progression of lens opacity in anesthetized C57BL/6 N mice. Anaesthesia dose and IR light exposure did not strongly influence lens opacity formation. Furthermore, eyes with corneal dryness-induced lens opacity recovered to normal status without additional intervention.ko_KR
dc.description.sponsorshipThis research was supported by Korea Mouse Phenotyping Project (NRF2013M3A9D5072551) of the Ministry of Science and ICT through the National Research Foundation.ko_KR
dc.language.isoenko_KR
dc.publisherBMCko_KR
dc.subjectLens opacity-
dc.subjectAnaesthetic dose-
dc.subjectOcular surface dryness-
dc.subjectInfrared light-
dc.subjectC57BL/6 N mice-
dc.titleEvaluation of factors related to Anaesthesia-induced Lens opacity in experimental miceko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이훈-
dc.contributor.AlternativeAuthor김홍경-
dc.contributor.AlternativeAuthor신해솔-
dc.contributor.AlternativeAuthor한수정-
dc.contributor.AlternativeAuthor윤상철-
dc.contributor.AlternativeAuthor성제경-
dc.contributor.AlternativeAuthor서경율-
dc.citation.journaltitleLaboratory Animal Researchko_KR
dc.language.rfc3066en-
dc.rights.holderThe Author(s)-
dc.date.updated2020-01-12T04:47:00Z-
dc.citation.number1ko_KR
dc.citation.startpage1ko_KR
dc.citation.volume36ko_KR
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