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Transduction of artificial transcriptional regulatory proteins into human cells

Cited 14 time in Web of Science Cited 19 time in Scopus
Authors

Yun, Chae-Ok; Shin, Hyun-Chul; Kim, Tae-Dong; Yoon, Wan-Hee; Kang, Yoon-A; Kwon, Heung-Sun; Kim, Seong Keun; Kim, Jin-Soo

Issue Date
2008-09
Publisher
Oxford University Press
Citation
Nucleic Acids Research, Vol.36 No.16, p. e103
Abstract
Protein transduction (PT) is a method for delivering proteins into mammalian cells. PT is accomplished by linking a small peptide tag-called a PT domain (PTD)to a protein of interest, which generates a functional fusion protein that can penetrate efficiently into mammalian cells. In order to study the functions of a transcription factor (TF) of interest, expression plasmids that encode the TF often are transfected into mammalian cells. However, the efficiency of DNA transfection is highly variable among different cell types and is usually very low in primary cells, stem cells and tumor cells. Zinc-finger transcription factors (ZF-TFs) can be tailor-made to target almost any gene in the human genome. However, the extremely low efficiency of DNA transfection into cancer cells, both in vivo and in vitro, limits the utility of ZF-TFs. Here, we report on an artificial ZF-TF that has been fused to a well-characterized PTD from the human immunodeficiency virus-1 (HIV-1) transcriptional activator protein, Tat. This ZF-TF targeted the endogenous promoter of the human VEGF-A gene. The PTD-attached ZF-TF was delivered efficiently into human cells in vitro. In addition, the VEGF-A-specific transcriptional repressor retarded the growth rate of tumor cells in a mouse xenograft experiment.
ISSN
0305-1048
URI
https://hdl.handle.net/10371/165612
DOI
https://doi.org/10.1093/nar/gkn398
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  • College of Natural Sciences
  • Department of Chemistry
Research Area Biology and Biochemistry

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