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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Cited 1,042 time in Web of Science Cited 1,095 time in Scopus
Authors
Cho, Seung Woo; Kim, Sojung; Kim, Jong Min; Kim, Jin-Soo
Issue Date
2013-03
Citation
Nature Biotechnology, Vol.31 No.3, pp.230-232
Abstract
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
ISSN
1087-0156
URI
http://hdl.handle.net/10371/165629
DOI
https://doi.org/10.1038/nbt.2507
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