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Fine-Tuning Next-Generation Genome Editing Tools

Cited 29 time in Web of Science Cited 30 time in Scopus
Authors
Kanchiswamy, Chidananda Nagamangala; Maffei, Massimo; Malnoy, Mickael; Velasco, Riccardo; Kim, Jin-Soo
Issue Date
2016-07
Citation
Trends in Biotechnology, Vol.34 No.7, pp.562-574
Keywords
CRISPR/Cas9Genome editingOff-targetOn-targetTALENsTarget specificityZFNs
Abstract
The availability of genome sequences of numerous organisms and the revolution brought about by genome editing tools (e.g., ZFNs, TALENs, and CRISPR/Cas9 or RGENs) has provided a breakthrough in introducing targeted genetic changes both to explore emergent phenotypes and to introduce new functionalities. However, the wider application of these tools in biology, agriculture, medicine, and biotechnology is limited by off-target mutation effects. In this review, we compare available methods for detecting, measuring, and analyzing off-target mutations. Furthermore, we particularly focus on CRISPR/Cas9 regarding various methods, tweaks, and software tools available to nullify off-target effects.
ISSN
0167-7799
URI
http://hdl.handle.net/10371/165665
DOI
https://doi.org/10.1016/j.tibtech.2016.03.007
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