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dCas9-mediated nanoelectrokinetic direct detection of target gene for liquid biopsy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hyomin | - |
dc.contributor.author | Choi, Jihye | - |
dc.contributor.author | Jeong, Euihwan | - |
dc.contributor.author | Baek, Seongho | - |
dc.contributor.author | Kim, Hee Chan | - |
dc.contributor.author | Chae, Jong-Hee | - |
dc.contributor.author | Koh, Youngil | - |
dc.contributor.author | Seo, Sang Woo | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Kim, Sung Jae | - |
dc.date.accessioned | 2020-04-27T12:56:50Z | - |
dc.date.available | 2020-04-27T12:56:50Z | - |
dc.date.created | 2019-08-21 | - |
dc.date.created | 2019-08-21 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.citation | Nano Letters, Vol.18 No.12, pp.7642-7650 | - |
dc.identifier.issn | 1530-6984 | - |
dc.identifier.other | 81593 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165693 | - |
dc.description.abstract | The-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapid and direct detection. Theoretical analysis on ICP phenomenon figured out a critical mobility, elucidating two distinguishable concentrating behaviors near a nanojunction, a stacking and a propagating behavior. Through the modulation of the critical mobility to shift those behaviors, the C-C chemokine receptor type 5 (CCR5) sequences were optically detected without PCR amplification. Conclusively, the proposed dCas9-mediated genetic detection methodology based on ICP would provide rapid and accurate micro/nanofluidic platform of liquid biopsies for disease diagnostics. | - |
dc.language | 영어 | - |
dc.publisher | American Chemical Society | - |
dc.title | dCas9-mediated nanoelectrokinetic direct detection of target gene for liquid biopsy | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.contributor.AlternativeAuthor | 서상우 | - |
dc.contributor.AlternativeAuthor | 김성재 | - |
dc.contributor.AlternativeAuthor | 채종희 | - |
dc.contributor.AlternativeAuthor | 김희찬 | - |
dc.identifier.doi | 10.1021/acs.nanolett.8b03224 | - |
dc.citation.journaltitle | Nano Letters | - |
dc.identifier.wosid | 000453488800029 | - |
dc.identifier.scopusid | 2-s2.0-85058082931 | - |
dc.citation.endpage | 7650 | - |
dc.citation.number | 12 | - |
dc.citation.startpage | 7642 | - |
dc.citation.volume | 18 | - |
dc.identifier.sci | 000453488800029 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Hee Chan | - |
dc.contributor.affiliatedAuthor | Chae, Jong-Hee | - |
dc.contributor.affiliatedAuthor | Seo, Sang Woo | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.contributor.affiliatedAuthor | Kim, Sung Jae | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CONCENTRATION POLARIZATION | - |
dc.subject.keywordPlus | DNA RECOGNITION | - |
dc.subject.keywordPlus | ELECTROKINETIC TRANSPORT | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | PRECONCENTRATION | - |
dc.subject.keywordPlus | ELECTROPHORESIS | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | MOBILITY | - |
dc.subject.keywordAuthor | Ion concentration polarization | - |
dc.subject.keywordAuthor | selective preconcentration | - |
dc.subject.keywordAuthor | dCas9 | - |
dc.subject.keywordAuthor | liquid biopsy | - |
dc.subject.keywordAuthor | micro/nanofluidics | - |
dc.subject.keywordAuthor | direct detection | - |
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