Publications
Detailed Information
CRISPR RNAs trigger innate immune responses in human cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sojung | - |
dc.contributor.author | Koo, Taeyoung | - |
dc.contributor.author | Jee, Hyeon-Gun | - |
dc.contributor.author | Cho, Hee-Yeon | - |
dc.contributor.author | Lee, Gyeorae | - |
dc.contributor.author | Lim, Dong-Gyun | - |
dc.contributor.author | Shin, Hyoung Shik | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2020-04-27T13:01:22Z | - |
dc.date.available | 2020-04-27T13:01:22Z | - |
dc.date.created | 2019-06-11 | - |
dc.date.created | 2019-06-11 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.citation | Genome Research, Vol.28 No.3, pp.367-373 | - |
dc.identifier.issn | 1088-9051 | - |
dc.identifier.other | 75188 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165708 | - |
dc.description.abstract | Here, we report that CRISPR guide RNAs (gRNAs) with a 5'-triphosphate group (5'-ppp gRNAs) produced via in vitro transcription trigger RNA-sensing innate immune responses in human and murine cells, leading to cytotoxicity. 5'-ppp gRNAs in the cytosol are recognized by DDX58, which in turn activates type I interferon responses, causing up to similar to 80% cell death. We show that the triphosphate group can be removed by a phosphatase in vitro and that the resulting St-hydroxyl gRNAs in complex with Cas9 or Cpfl avoid innate immune responses and can achieve targeted mutagenesis at a frequency of 95% in primary human CD4(+) T cells. These results are in line with previous findings that chemically synthesized sgRNAs with a 5'-hydroxyl group are much more efficient than in vitro-transcribed (IVT) sgRNAs in human and other mammalian cells. The phosphatase treatment of IVT sgRNAs is a cost-effective method for making highly active sgRNAs, avoiding innate immune responses in human cells. | - |
dc.language | 영어 | - |
dc.publisher | Cold Spring Harbor Laboratory Press | - |
dc.title | CRISPR RNAs trigger innate immune responses in human cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1101/gr.231936.117 | - |
dc.citation.journaltitle | Genome Research | - |
dc.identifier.wosid | 000426355600009 | - |
dc.identifier.scopusid | 2-s2.0-85046097277 | - |
dc.citation.endpage | 373 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 367 | - |
dc.citation.volume | 28 | - |
dc.identifier.sci | 000426355600009 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RIBONUCLEOPROTEIN COMPLEXES | - |
dc.subject.keywordPlus | CAS9 RIBONUCLEOPROTEINS | - |
dc.subject.keywordPlus | GUIDED ENDONUCLEASE | - |
dc.subject.keywordPlus | GENE KNOCKOUT | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | CPF1 | - |
dc.subject.keywordPlus | EMBRYOS | - |
dc.subject.keywordPlus | MICE | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.