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Transient receptor potential vanilloid-1 mediates heat-shock-induced matrix metalloproteinase-1 expression in human epidermal keratinocytes

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dc.contributor.authorLi, Wen H-
dc.contributor.authorLee, Young M-
dc.contributor.authorKim, Jee Y-
dc.contributor.authorKang, Seokwon-
dc.contributor.authorKim, Sangmin-
dc.contributor.authorKim, Kyu H-
dc.contributor.authorPark, Chi-Hyun-
dc.contributor.authorChung, Jin H-
dc.date.accessioned2009-11-27T04:54:59Z-
dc.date.available2009-11-27T04:54:59Z-
dc.date.issued2007-05-18-
dc.identifier.citationJ Invest Dermatol. 2007 Oct;127(10):2328-35. Epub 2007 May 17.en
dc.identifier.issn1523-1747 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17508023-
dc.identifier.urihttp://hdl.handle.net/10371/16591-
dc.description.abstractTransient receptor potential vanilloid-1 (TRPV1), a heat-gated channel, was recently found on human keratinocytes and the activation of epidermal TRPV1 was known to induce release of proinflammatory mediators. However, the functional consequences of TRPV1 activation in cutaneous physiology and pathology have not been elucidated clearly. In this study, we investigated the role of TRPV1 on the matrix metalloproteinase (MMP)-1 expression induced by heat shock in human epidermal keratinocytes. Heat shock induced the expression of MMP-1 mRNA and protein in a temperature-dependent manner in an immortalized human keratinocyte cell line (HaCaT) and normal human epidermal keratinocytes (NHK). Heat-shock-induced MMP-1 expression was decreased by treatment of the TRPV1 inhibitors (capsazepine and ruthenium red) or knockdown of TRPV1 using RNA interference in HaCaT cells. Overexpression of TRPV1 greatly increased heat-shock-induced MMP-1 promoter activity in HEK 293 cells. Furthermore, direct activation of TRPV1 by capsaicin, a TRPV1 agonist, increased MMP-1 expression. We found that heat shock induced calcium influx through TRPV1 and that extracellular calcium was necessary for heat-shock-induced MMP-1 expression in HaCaT cells. Taken together, our results suggest that heat-shock-induced MMP-1 expression is mediated by activation of TRPV1 and is dependent on a calcium-dependent signaling process in human epidermal keratinocytes.en
dc.language.isoen-
dc.publisherNature Publishing Groupen
dc.subjectCalcium Signaling/physiologyen
dc.subjectCapsaicin/analogs & derivatives/pharmacologyen
dc.subjectCell Lineen
dc.subjectCells, Cultureden
dc.subjectGene Expression Regulation/drug effects/physiologyen
dc.subjectHot Temperatureen
dc.subjectHumansen
dc.subjectKeratinocytes/*metabolism/pathologyen
dc.subjectMaleen
dc.subjectMatrix Metalloproteinase 1/genetics/*metabolismen
dc.subjectRNA, Messenger/genetics/metabolismen
dc.subjectRNA, Small Interfering/pharmacologyen
dc.subjectRuthenium Red/pharmacologyen
dc.subjectTRPV Cation Channels/antagonists & inhibitors/genetics/*metabolismen
dc.titleTransient receptor potential vanilloid-1 mediates heat-shock-induced matrix metalloproteinase-1 expression in human epidermal keratinocytesen
dc.typeArticleen
dc.contributor.AlternativeAuthor강석원-
dc.contributor.AlternativeAuthor김상민-
dc.contributor.AlternativeAuthor박지현-
dc.identifier.doi10.1038/sj.jid.5700880-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dermatology (피부과학전공)Journal Papers (저널논문_피부과학전공)
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