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In Situ Quantification and Profiling of Phosphatidylcholine in Mouse Brain Tissue by Matrix-assisted Laser Desorption Ionization with a Liquid Matrix

Cited 2 time in Web of Science Cited 3 time in Scopus
Authors
Park, Kyung Man; Hyeon, Taeghwan; Kim, Myung Soo; Moon, Jeong Hee
Issue Date
2017-06
Citation
Bulletin of the Korean Chemical Society, Vol.38 No.6, pp.636-641
Keywords
MALDIQuantitative profilingPhosphatidylcholineMouse brain tissue
Abstract
A method based on the analyte-to-matrix ion abundance ratio in the matrix-assisted laser desorption ionization (MALDI) spectrum was used for the quantification of phosphatidylcholine (PC) in mouse brain tissue. The fact that the tissue was heavily contaminated and hence that the matrix suppression was unacceptably high forbade the in situ quantification of PC. We eliminated most of the polar contaminants, and some nonpolar ones as well, by washing the tissue with water. In the process, we observed the presence of two different populations of PC, one washable in water and the other unwashable. Finally, we present a quantitative profile of the unwashable PC in a mouse brain tissue evaluated from its MALDI spectral data.
ISSN
0253-2964
URI
http://hdl.handle.net/10371/165953
DOI
https://doi.org/10.1002/bkcs.11142
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Chemical Convergence for Energy and Environment (에너지환경 화학융합기술전공)Journal Papers (저널논문_에너지환경 화학융합기술전공)
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