Jannaschia seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea

Abstract

A slightly halophilic alphaproteoloacterium, designated CL-SP26(T), was isolated from hypersaline water of a solar saltern located in Seosin, Korea. 16S rRNA gene sequence analysis revealed an affiliation with the genus Jannaschia. Sequence similarities between CL-SP26(T) and type strains of members of the genus Jannaschia were 94.9-97.2%. Cells were rod-shaped and motile with one or more monopolar flagella. Strain CL-SP26(T) grew on solid media as circular red colonies. It was able to grow in 3-10% sea salt; however, no growth occurred in media containing NaCl as the only salt. Strain CL-SP26(T) grew at 5-35 degrees C and pH 7-10. The major fatty acids were 18:1 omega 7c (64%) and 18: 0 (12.0%). Three fatty acids, 3-OH 14:0/iso 16: 1 (3.6%), 18:3 omega 6c (2.2%) and 10-methyl 19: 0 (1.9%), found in minor quantities in CL-SP26(T), are unique among Jannaschia species. The DNA G + C content was 63 mol%. According to physiological data, fatty acid composition and 16S rRNA gene sequence analysis, CL-SP26(T) was assigned to the genus Jannaschia, but could be distinguished from recognized species of the genus. Strain CL-SP26(T) (=KCCM 42114(T)=JCM 13035(T)) therefore represents a novel species, for which the name Jannaschia seosinensis sp. nov. is proposed.