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Screening of essential genes in Staphylococcus aureus N315 using comparative genomics and allelic replacement mutagenesis

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Authors
Ko, Kwan Soo; Lee, Ji Young; Song, Jae-Hoon; Baek, Jin Yang; Oh, Won Sup; Chun, Jongsik; Yoon, Ha Sik
Issue Date
2006-04
Citation
Journal of Microbiology and Biotechnology, Vol.16 No.4, pp.623-632
Keywords
Staphylococcus aureusessential genesgenomicsallelic replacement mutagenesisnew antimicrobial agent
Abstract
To find potential targets of novel antimicrobial agents, we identified essential genes of Staphylococcus aureus N315 by using comparative genomics and allele replacement mutagenesis. By comparing the genome of S. aureus N315 with those of Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pneumoniae, a total of 481 candidate target genes with similar amino acid sequences with at least three other species by > 40% sequence identity were selected. Of 481 disrupted candidate genes, 122 genes were identified as essential genes for growth of S. aureus N315. Of these, 5 1 essential genes were those not identified in any bacterial species, and 24 genes encode proteins of unknown function. Seventeen genes were determined as non-essential although they were identified as essential genes in other strain of S. aureus and other species. We found no significant difference among essential genes between Streptococcus pneumoniae and S. aureus with regard to cellular function.
ISSN
1017-7825
URI
http://hdl.handle.net/10371/166039
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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