S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Journal Papers (저널논문_화학생물공학부)
Endocytosis, intracellular transport, and exocytosis of lanthanide-doped upconverting nanoparticles in single living cells
- Bae, Yun Mi; Park, Yong Il; Nam, Sang Hwan; Kim, Jeong Hyun; Lee, Kyunghee; Kim, Hyung Min; Yoo, Byeongjun; Choi, Joon Sig; Lee, Kang Taek; Hyeon, Taeghwan; Suh, Yung Doug
- Issue Date
- Biomaterials, Vol.33 No.35, pp.9080-9086
- Lanthanide-doped upconverting nanoparticles (UCNPs) have recently attracted enormous attention in the field of biological imaging owing to their unique optical properties (near-infrared excitation followed by photoluminescence in the visible spectral range). For biological applications, it is critical to understand the interaction between these nanoparticles and biological systems at the cellular level. In this paper, using epi-fluorescence microscopy with 980-nm excitation, a full intracellular pathway composed of endocytosis, active transport, and exocytosis was clearly visualized for PEG-phospholipid-coated UCNPs in single HeLa cells, which was experimentally feasible mostly thanks to the excellent photostability and low cytotoxicity thereof. Each step in the pathway was characterized and identified by various chemical inhibition studies and spectroscopic measurements. (C) 2012 Elsevier Ltd. All rights reserved.
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- College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Chemical Convergence for Energy and Environment (에너지환경 화학융합기술전공)Journal Papers (저널논문_에너지환경 화학융합기술전공)