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Endocytosis, intracellular transport, and exocytosis of lanthanide-doped upconverting nanoparticles in single living cells

Cited 66 time in Web of Science Cited 71 time in Scopus
Authors
Bae, Yun Mi; Park, Yong Il; Nam, Sang Hwan; Kim, Jeong Hyun; Lee, Kyunghee; Kim, Hyung Min; Yoo, Byeongjun; Choi, Joon Sig; Lee, Kang Taek; Hyeon, Taeghwan; Suh, Yung Doug
Issue Date
2012-12
Citation
Biomaterials, Vol.33 No.35, pp.9080-9086
Keywords
NanoparticleImage analysisFluorescenceMolecular imagingCytotoxicity
Abstract
Lanthanide-doped upconverting nanoparticles (UCNPs) have recently attracted enormous attention in the field of biological imaging owing to their unique optical properties (near-infrared excitation followed by photoluminescence in the visible spectral range). For biological applications, it is critical to understand the interaction between these nanoparticles and biological systems at the cellular level. In this paper, using epi-fluorescence microscopy with 980-nm excitation, a full intracellular pathway composed of endocytosis, active transport, and exocytosis was clearly visualized for PEG-phospholipid-coated UCNPs in single HeLa cells, which was experimentally feasible mostly thanks to the excellent photostability and low cytotoxicity thereof. Each step in the pathway was characterized and identified by various chemical inhibition studies and spectroscopic measurements. (C) 2012 Elsevier Ltd. All rights reserved.
ISSN
0142-9612
URI
http://hdl.handle.net/10371/166217
DOI
https://doi.org/10.1016/j.biomaterials.2012.08.039
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Chemical Convergence for Energy and Environment (에너지환경 화학융합기술전공)Journal Papers (저널논문_에너지환경 화학융합기술전공)
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