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Ribonuclease S‐peptide as a carrier in fusion proteins
Cited 179 time in
Web of Science
Cited 188 time in Scopus
- Authors
- Issue Date
- 1993-03
- Publisher
- Cold Spring Harbor Laboratory Press
- Citation
- Protein Science, Vol.2 No.3, pp.348-356
- Abstract
- S-peptide (residues 1-20) and S-protein (residues 21-124) are the enzymatically inactive products of the limited digestion of ribonuclease A by subtilisin. S-peptide binds S-protein with high affinity to form ribonuclease S, which has full enzymatic activity. Recombinant DNA technology was used to produce a fusion protein having three parts: carrier, spacer, and target. The two carriers used were the first 15 residues of S-peptide (S15) and a mutant S15 in which Asp 14 had been changed to Asn (D14N S15). The spacer consisted of three proline residues and a four-residue sequence recognized by factor X(a) protease. The target was beta-galactosidase. The interaction between the S-peptide portion of the fusion protein and immobilized S-protein allowed for affinity purification of the fusion protein under denaturing (S15 as carrier) or nondenaturing (D14N S 1 5 as carrier) conditions. A sensitive method was developed to detect the fusion protein after sodium dodecyl sulfate-polyacrylamide gel electrophoresis by its ribonuclease activity following activation with S-protein. S-peptide has distinct advantages over existing carriers in fusion proteins in that it combines a small size (greater-than-or-equal-to 15 residues), a tunable affinity for ligand (K(d) greater-than-or-equal-to 10(-9) M), and a high sensitivity of detection (greater-than-or-equal-to 10(-16) mol in a gel).
- ISSN
- 0961-8368
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