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Transcriptome profiling of pluripotent pig embryonic stem cells originating from uni- and biparental embryos

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Authors
Choi, Kwang-Hwan; Lee, Dong-Kyung; Oh, Jong-Nam; Kim, Seung-Hun; Lee, Mingyun; Kim, Sung Woo; Lee, Chang-Kyu
Issue Date
2020-03-11
Publisher
BMC
Citation
BMC Research Notes. 2020 Mar 11;13(1):144
Keywords
Pig embryonic stem cellsRNA-seqTranscriptomeIn vitro fertilized embryoParthenogenetic embryoPreimplantation embryo
Abstract
Objectives
Pig pluripotent stem cells have tremendous potential because the pig is a valuable animal as both an agricultural resource and as a preclinical model of human therapy. To date, a lack of understanding of pig pluripotency has inhibited the derivation of embryonic stem cells (ESCs) and transgene-free induced pluripotent stem cells. Therefore, there has been no accessible or reliable transcriptome data for researching the genuine pig pluripotency network. Our previous study isolated authentic pig ESCs, which had teratoma-forming and direct differentiation ability, that were derived by activating the FGF2, ACTIVIN A, and WNT pathways. Here, we aimed to provide detailed information on transcriptome data of the newly derived pig ESCs and perform a comparative analysis with pig preimplantation embryo transcriptomes in a public database.

Data description
The transcriptome data of ESCs derived from in vitro fertilized and parthenogenetic embryos were generated by HiSeq 2500. Then, differentially expressed genes (DEGs) from each sample were compared with fibroblasts, and gene expression profiling was carried out for comparative analysis. Our data, as the first transcriptome dataset for genuine pig pluripotent cells, could be a general reference for explaining the molecular mechanism of species-specific pluripotency and improving understanding of the embryo development of domestic animals.
ISSN
1756-0500
Language
English
URI
http://hdl.handle.net/10371/168781
DOI
doi.org/10.1186/s13104-020-04987-6
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College of Agriculture and Life Sciences (농업생명과학대학)Program in Agricultural Biotechnology (협동과정-농업생물공학전공)Journal Papers (저널논문_협동과정-농업생물공학전공)
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