S-Space College of Medicine/School of Medicine (의과대학/대학원) Internal Medicine (내과학전공) Journal Papers (저널논문_내과학전공)
Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts
- Kim, Sang Wan; Her, Sun Ju; Kim, Seong Yeon; Shin, Chan Soo
- Issue Date
- Biochem Biophys Res Commun. 2005 Feb 18;327(3):811-9.
- 1-Methyl-3-isobutylxanthine/pharmacology; 3T3 Cells; Adipocytes/*metabolism; Alkaline Phosphatase/metabolism; Animals; Base Sequence; CCAAT-Enhancer-Binding Protein-alpha/metabolism; Cell Differentiation/*physiology; Cells, Cultured; Dexamethasone/pharmacology; Gene Expression Regulation; Insulin/metabolism; Mice; Osteoblasts/*cytology; PPAR gamma/metabolism; Phenotype; Transcription Factors/genetics/*physiology
- Osteoblasts and adipocytes originate from common mesenchymal progenitor cells. We have investigated whether mouse osteoblastic MC3T3-E1 cells can be induced to transdifferentiate into mature adipocytes by the ectopic expression of adipogenic transcription factors, PPARgamma, C/EBPalpha, or both. Retrovirus-mediated overexpression of PPARgamma alone or both PPARgamma and C/EBPalpha resulted in reduced alkaline phosphatase activity and osteoblast-specific gene expression. Moreover, foci of adipocytes were identified in conditions favoring osteoblastic maturation. Upon treatment with insulin, dexamethasone, and IBMX, cells overexpressing PPARgamma alone or both PPARgamma and C/EBPalpha showed marked transdifferentiation to mature adipocytes expressing molecular markers of adipocytes. Cells expressing both PPARgamma and C/EBPalpha showed more robust phenotype of adipocytes than the cells expressing PPARgamma alone. Overexpression of C/EBPalpha alone did not result in adipogenesis. These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes.
- 0006-291X (Print)
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