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CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell

Cited 12 time in Web of Science Cited 14 time in Scopus
Authors
Hur, D. Y.; Lee, M. H.; Kim, J. W.; Kim, J. H.; Shin, Y. K.; Rho, J. K.; Kwack, K. B.; Lee, W. J.; Han, B. G.
Issue Date
2005-02-01
Publisher
Wiley-Blackwell
Citation
Cell Prolif. 2005 Feb;38(1):35-45.
Keywords
Antigens, CD19/*biosynthesisB-Lymphocytes/*cytology/metabolismCell LineCell Membrane/metabolismCell SeparationCell Transformation, ViralEpstein-Barr Virus Nuclear Antigens/metabolismFlow CytometryHerpesvirus 4, Human/*metabolismHumansLeukocytes, Mononuclear/metabolismLymphocytes/virologyPhenotypeReverse Transcriptase Polymerase Chain Reaction*Signal TransductionTime FactorsViral Matrix Proteins/metabolismViral Proteins
Abstract
Epstein-Barr virus (EBV) infection in vitro immortalizes primary B cells and generates B lymphoblastoid cell lines (LCLs). These EBV-LCLs have been used for several purposes in immunological and genetic studies, but some trials involving these transformations fail for unknown reasons, and several EBV-LCLs do not grow in normal culture. In this study, we improved the immortalization method by CD19 and B-cell receptor (BCR) co-ligation. This method shortens the time required for the immortalization and generation of EBV-LCLs but does not alter the cell phenotype of the LCLs nor the expression of the EBV genes. In particular, the CD19 and BCR co-ligation method was found to be the most effective method examined. EBV-infected B cells induced by CD19 and/or BCR ligation expressed the intracellular latent membrane protein LMP-1 earlier than EBV-infected B cells, and the expression of intracellular LMP-1 was found to be closely related to the time of immortalization. These results suggest that the modified method, using CD19 and/or BCR ligation, may efficiently generate EBV-LCLs, by expressing intracellular LMP-1 at an early stage.
ISSN
0960-7722 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15679865

http://hdl.handle.net/10371/22639
DOI
https://doi.org/10.1111/j.1365-2184.2005.00328.x
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College of Medicine/School of Medicine (의과대학/대학원)Immunology (면역학전공)Journal Papers (저널논문_면역학전공)
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