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CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hur, D. Y. | - |
dc.contributor.author | Lee, M. H. | - |
dc.contributor.author | Kim, J. W. | - |
dc.contributor.author | Kim, J. H. | - |
dc.contributor.author | Shin, Y. K. | - |
dc.contributor.author | Rho, J. K. | - |
dc.contributor.author | Kwack, K. B. | - |
dc.contributor.author | Lee, W. J. | - |
dc.contributor.author | Han, B. G. | - |
dc.date.accessioned | 2009-12-24T11:52:46Z | - |
dc.date.available | 2009-12-24T11:52:46Z | - |
dc.date.issued | 2005-02-01 | - |
dc.identifier.citation | Cell Prolif. 2005 Feb;38(1):35-45. | en |
dc.identifier.issn | 0960-7722 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15679865 | - |
dc.identifier.uri | https://hdl.handle.net/10371/22639 | - |
dc.description.abstract | Epstein-Barr virus (EBV) infection in vitro immortalizes primary B cells and generates B lymphoblastoid cell lines (LCLs). These EBV-LCLs have been used for several purposes in immunological and genetic studies, but some trials involving these transformations fail for unknown reasons, and several EBV-LCLs do not grow in normal culture. In this study, we improved the immortalization method by CD19 and B-cell receptor (BCR) co-ligation. This method shortens the time required for the immortalization and generation of EBV-LCLs but does not alter the cell phenotype of the LCLs nor the expression of the EBV genes. In particular, the CD19 and BCR co-ligation method was found to be the most effective method examined. EBV-infected B cells induced by CD19 and/or BCR ligation expressed the intracellular latent membrane protein LMP-1 earlier than EBV-infected B cells, and the expression of intracellular LMP-1 was found to be closely related to the time of immortalization. These results suggest that the modified method, using CD19 and/or BCR ligation, may efficiently generate EBV-LCLs, by expressing intracellular LMP-1 at an early stage. | en |
dc.language.iso | en | en |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Antigens, CD19/*biosynthesis | en |
dc.subject | B-Lymphocytes/*cytology/metabolism | en |
dc.subject | Cell Line | en |
dc.subject | Cell Membrane/metabolism | en |
dc.subject | Cell Separation | en |
dc.subject | Cell Transformation, Viral | en |
dc.subject | Epstein-Barr Virus Nuclear Antigens/metabolism | en |
dc.subject | Flow Cytometry | en |
dc.subject | Herpesvirus 4, Human/*metabolism | en |
dc.subject | Humans | en |
dc.subject | Leukocytes, Mononuclear/metabolism | en |
dc.subject | Lymphocytes/virology | en |
dc.subject | Phenotype | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | Time Factors | en |
dc.subject | Viral Matrix Proteins/metabolism | en |
dc.subject | Viral Proteins | en |
dc.subject | Signal Transduction | - |
dc.title | CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell | en |
dc.type | Article | en |
dc.identifier.doi | 10.1111/j.1365-2184.2005.00328.x | - |
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