Regulation of UVB-induced IL-8 and MCP-1 production in skin keratinocytes by increasing vitamin C uptake via the redistribution of SVCT-1 from the cytosol to the membrane

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Kang, Jae Seung; Kim, Ha Na; Jung, Da Jung; Kim, Jee Eun; Mun, Ga Hee; Kim, Yeong Seok; Cho, Daeho; Shin, Dong Hoon; Hwang, Young-Il; Lee, Wang Jae
Issue Date
Nature Publishing Group
J Invest Dermatol 127:698-706
Antioxidants/pharmacologyAscorbic Acid/*metabolismCell Line, TumorCell Membrane/*metabolismCytosol/*metabolismHumansInterleukin-8/*biosynthesisKeratinocytes/*metabolismMAP Kinase Signaling SystemOrganic Anion Transporters, Sodium-Dependent/*biosynthesisProtein TransportReactive Oxygen SpeciesReceptors, CCR2Receptors, Chemokine/*biosynthesisSkin/metabolismSymporters/*biosynthesis*Ultraviolet Rays
It is well known that UVB (290-320 nm) induces inflammation in skin by the transcription and release of cytokines and chemokines from skin keratinocytes. In addition, it is considered that intracellular reactive oxygen species (ROS) plays an important role in UVB-induced inflammatory response in the skin. Therefore, we investigated the effect of vitamin C, a potent antioxidant, on the regulation of UVB-induced skin inflammation via the modulation of chemokines production. Vitamin C uptake into keratinocytes is increased by UVB irradiation in a time- and dose-dependent manner through the translocation of sodium-dependent vitamin C transporter-1 (SVCT-1), a vitamin C-specific transporter, from the cytosol to the membrane. To evaluate the effect of vitamin C on the chemokine mRNA expression, we performed RNase protection assay. As a result, there was a remarkable change in chemokine mRNA expression, especially IL-8 and monocyte chemoattractant protein (MCP)-1 expression. In addition, increased IL-8 and MCP-1 mRNA expressions were suppressed by vitamin C treatment. We also confirmed the results of protein levels measured by ELISA. Taken together, vitamin C uptake is increased in UVB-irradiated keratinocytes through the translocation of SVCT-1 and regulates inflammatory response in the skin via the downregulation of IL-8 and MCP-1 production.
1523-1747 (Electronic)
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College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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