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Regulation of UVB-induced IL-8 and MCP-1 production in skin keratinocytes by increasing vitamin C uptake via the redistribution of SVCT-1 from the cytosol to the membrane

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dc.contributor.authorKang, Jae Seung-
dc.contributor.authorKim, Ha Na-
dc.contributor.authorJung, Da Jung-
dc.contributor.authorKim, Jee Eun-
dc.contributor.authorMun, Ga Hee-
dc.contributor.authorKim, Yeong Seok-
dc.contributor.authorCho, Daeho-
dc.contributor.authorShin, Dong Hoon-
dc.contributor.authorHwang, Young-Il-
dc.contributor.authorLee, Wang Jae-
dc.date.accessioned2009-12-30T01:37:41Z-
dc.date.available2009-12-30T01:37:41Z-
dc.date.issued2006-
dc.identifier.citationJ Invest Dermatol 127:698-706en
dc.identifier.issn1523-1747 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17008880-
dc.identifier.urihttp://hdl.handle.net/10371/23409-
dc.description.abstractIt is well known that UVB (290-320 nm) induces inflammation in skin by the transcription and release of cytokines and chemokines from skin keratinocytes. In addition, it is considered that intracellular reactive oxygen species (ROS) plays an important role in UVB-induced inflammatory response in the skin. Therefore, we investigated the effect of vitamin C, a potent antioxidant, on the regulation of UVB-induced skin inflammation via the modulation of chemokines production. Vitamin C uptake into keratinocytes is increased by UVB irradiation in a time- and dose-dependent manner through the translocation of sodium-dependent vitamin C transporter-1 (SVCT-1), a vitamin C-specific transporter, from the cytosol to the membrane. To evaluate the effect of vitamin C on the chemokine mRNA expression, we performed RNase protection assay. As a result, there was a remarkable change in chemokine mRNA expression, especially IL-8 and monocyte chemoattractant protein (MCP)-1 expression. In addition, increased IL-8 and MCP-1 mRNA expressions were suppressed by vitamin C treatment. We also confirmed the results of protein levels measured by ELISA. Taken together, vitamin C uptake is increased in UVB-irradiated keratinocytes through the translocation of SVCT-1 and regulates inflammatory response in the skin via the downregulation of IL-8 and MCP-1 production.en
dc.language.isoen-
dc.publisherNature Publishing Groupen
dc.subjectAntioxidants/pharmacologyen
dc.subjectAscorbic Acid/*metabolismen
dc.subjectCell Line, Tumoren
dc.subjectCell Membrane/*metabolismen
dc.subjectCytosol/*metabolismen
dc.subjectHumansen
dc.subjectInterleukin-8/*biosynthesisen
dc.subjectKeratinocytes/*metabolismen
dc.subjectMAP Kinase Signaling Systemen
dc.subjectOrganic Anion Transporters, Sodium-Dependent/*biosynthesisen
dc.subjectProtein Transporten
dc.subjectReactive Oxygen Speciesen
dc.subjectReceptors, CCR2en
dc.subjectReceptors, Chemokine/*biosynthesisen
dc.subjectSkin/metabolismen
dc.subjectSymporters/*biosynthesisen
dc.subject*Ultraviolet Raysen
dc.titleRegulation of UVB-induced IL-8 and MCP-1 production in skin keratinocytes by increasing vitamin C uptake via the redistribution of SVCT-1 from the cytosol to the membraneen
dc.typeArticleen
dc.contributor.AlternativeAuthor강재승-
dc.contributor.AlternativeAuthor김하나-
dc.contributor.AlternativeAuthor정다정-
dc.contributor.AlternativeAuthor김지은-
dc.contributor.AlternativeAuthor문가희-
dc.contributor.AlternativeAuthor김용석-
dc.contributor.AlternativeAuthor조대호-
dc.contributor.AlternativeAuthor신동훈-
dc.contributor.AlternativeAuthor황영일-
dc.contributor.AlternativeAuthor이왕재-
dc.identifier.doi10.1038/sj.jid.5700572-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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