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Generation of a rabbit V(H) domain antibody polyspecific to c-Met and adenoviral knob protein

Cited 3 time in Web of Science Cited 4 time in Scopus
Authors
Im, Shin-Young; Kim, Ki Su; Yun, Chae-Ok; Kim, Joo-Hang; Yi, Kye-Sook; Chung, Junho
Issue Date
2005-11-22
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2006 Jan 6;339(1):305-12. Epub 2005 Nov 15.
Keywords
Adenoviridae/*immunologyAmino Acid SequenceAnimals*Antibodies, BispecificHumansImmunoglobulin Heavy Chains/*immunologyImmunoglobulin Variable Region/*immunologyMolecular Sequence DataPeptide LibraryProto-Oncogene Proteins c-met/*immunologyRabbitsViral Proteins/*immunology
Abstract
Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein V(H) fragment linked with an anti-c-Met V(H)), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one V(H) domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells.
ISSN
0006-291X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16298341

http://hdl.handle.net/10371/23411
DOI
https://doi.org/10.1016/j.bbrc.2005.10.208
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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