S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Biochemistry & Molecular Biology (생화학교실) Journal Papers (저널논문_생화학교실)
Generation of a rabbit V(H) domain antibody polyspecific to c-Met and adenoviral knob protein
- Im, Shin-Young; Kim, Ki Su; Yun, Chae-Ok; Kim, Joo-Hang; Yi, Kye-Sook; Chung, Junho
- Issue Date
- Biochem Biophys Res Commun. 2006 Jan 6;339(1):305-12. Epub 2005 Nov 15.
- Adenoviridae/*immunology; Amino Acid Sequence; Animals; *Antibodies, Bispecific; Humans; Immunoglobulin Heavy Chains/*immunology; Immunoglobulin Variable Region/*immunology; Molecular Sequence Data; Peptide Library; Proto-Oncogene Proteins c-met/*immunology; Rabbits; Viral Proteins/*immunology
- Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein V(H) fragment linked with an anti-c-Met V(H)), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one V(H) domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells.
- 0006-291X (Print)
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