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Generation of a rabbit V(H) domain antibody polyspecific to c-Met and adenoviral knob protein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Im, Shin-Young | - |
dc.contributor.author | Kim, Ki Su | - |
dc.contributor.author | Yun, Chae-Ok | - |
dc.contributor.author | Kim, Joo-Hang | - |
dc.contributor.author | Yi, Kye-Sook | - |
dc.contributor.author | Chung, Junho | - |
dc.date.accessioned | 2009-12-30T01:42:27Z | - |
dc.date.available | 2009-12-30T01:42:27Z | - |
dc.date.issued | 2005-11-22 | - |
dc.identifier.citation | Biochem Biophys Res Commun. 2006 Jan 6;339(1):305-12. Epub 2005 Nov 15. | en |
dc.identifier.issn | 0006-291X (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16298341 | - |
dc.identifier.uri | https://hdl.handle.net/10371/23411 | - |
dc.description.abstract | Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein V(H) fragment linked with an anti-c-Met V(H)), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one V(H) domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Adenoviridae/*immunology | en |
dc.subject | Amino Acid Sequence | en |
dc.subject | Animals | en |
dc.subject | Humans | en |
dc.subject | Immunoglobulin Heavy Chains/*immunology | en |
dc.subject | Immunoglobulin Variable Region/*immunology | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Peptide Library | en |
dc.subject | Proto-Oncogene Proteins c-met/*immunology | en |
dc.subject | Rabbits | en |
dc.subject | Viral Proteins/*immunology | en |
dc.subject | Antibodies, Bispecific | - |
dc.title | Generation of a rabbit V(H) domain antibody polyspecific to c-Met and adenoviral knob protein | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 임신영 | - |
dc.contributor.AlternativeAuthor | 김기수 | - |
dc.contributor.AlternativeAuthor | 윤채옥 | - |
dc.contributor.AlternativeAuthor | 김주항 | - |
dc.contributor.AlternativeAuthor | 이계숙 | - |
dc.contributor.AlternativeAuthor | 정준호 | - |
dc.identifier.doi | 10.1016/j.bbrc.2005.10.208 | - |
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