RNA polymerase beta-subunit gene (rpoB) sequence analysis for the identification of Bacteroides spp

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Ko, K. S.; Kuwahara, T.; Haehwa, L.; Yoon, Y. J.; Kim, B. J.; Lee, K. H.; Ohnishi, Y.; Kook, Y. H.
Issue Date
Blackwell Publishing
Clin Microbiol Infect. 2007 Jan;13(1):48-54.
Bacterial Proteins/*geneticsBacteroides/*classificationBacteroides Infections/microbiologyDNA-Directed RNA Polymerases/*genetics*Genes, BacterialHumansMolecular Sequence DataSequence Analysis, ProteinSpecies Specificity
Partial rpoB sequences (317 bp) of 11 species of Bacteroides, two Porphyromonas spp. and two Prevotella spp. were compared to delineate the genetic relationships among Bacteroides and closely related anaerobic species. The high level of inter-species sequence dissimilarities (7.6-20.8%) allowed the various Bacteroides spp. to be distinguished. The position of the Bacteroides distasonis and Bacteriodes merdae cluster in the rpoB tree was different from the position in the 16S rRNA gene tree. Based on rpoB sequence similarity and clustering in the rpoB tree, it was possible to correctly re-identify 80 clinical isolates of Bacteroides. In addition to two subgroups, cfiA-negative (division I) and cfiA-positive (division II), of Bacteroides fragilis isolates, two distinct subgroups were also found among Bacteroides ovatus and Bacteroides thetaiotaomicron isolates. Bacteroides genus-specific rpoB PCR and B. fragilis species-specific rpoB PCR allowed Bacteroides spp. to be differentiated from Porphyromonas and Prevotella spp., and also allowed B. fragilis to be differentiated from other non-fragilisBacteroides spp. included in the present study.
1198-743X (Print)
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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