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Change of proliferation rate of corneal epithelium in the rabbit with orthokeratology lens

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Authors
Shin, Young Joo; Kim, Mee Kum; Wee, Won Ryang; Lee, Jin Hak; Shin, Dong-Bin; Lee, Jae Lim; Xu, Yong Gen; Choi, Si-Whan
Issue Date
2005
Publisher
Karger
Citation
Ophthalmic Res 2005;37:94-103
Keywords
AnimalsBromodeoxyuridine/metabolismCell Count*Cell Proliferation*Contact LensesEpithelium, Corneal/metabolism/*pathologyProsthesis FittingRabbits
Abstract
OBJECTIVE: To investigate the cell proliferation rate of normal corneal epithelium with extended orthokeratology lens (OKL) wear in comparison with extended rigid gas-permeable (RGP) lens wear. METHODS: Twenty-four rabbits were fitted unilaterally with either an OKL or an RGP lens, and the other eye was used as a control. They were injected with 5-bromo-2-deoxyuridine (BrdU) 24 h prior to being sacrificed. The rabbits were sacrificed at 1, 3, 7 and 14 days after lens fitting. The cornea from the superior limbus to the center was taken at 1.0-mm intervals. The BrdU-labeled cells were counted in medium power fields (x200) in each sample using light microscopy. RESULTS: The number of BrdU-labeled cells in the RGP lens group initially increased, but the number decreased in the corneal center and superior limbus by 32 and 8%, respectively, after 14 days. There was no statistically significant change. However, the number of BrdU-labeled cells in the OKL group decreased after 3 days, and the number of BrdU-labeled cells was reduced in the center and superior limbus by 63 and 8%, respectively, after 14 days. The change in proliferation in the corneal center in the OKL-wearing rabbits was statistically significant compared to the control (p < 0.05). CONCLUSIONS: Wearing an OKL had a greater effect on the change of the proliferation pattern in the epithelium than wearing an RGP lens, which suggests that the OKL might be less physiologic than the RGP lens is.
ISSN
0030-3747 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15746565

http://hdl.handle.net/10371/23429
DOI
https://doi.org/10.1159/000084251
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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