Browse

NOX4 as an oxygen sensor to regulate TASK-1 activity

Cited 86 time in Web of Science Cited 88 time in Scopus
Authors
Lee, Young-Mee; Kim, Byung-Joo; Chun, Yang-Sook; So, Insuk; Choi, Hyunsung; Kim, Myung-Suk; Park, Jong-Wan
Issue Date
2006-07-16
Publisher
Elsevier
Citation
Cell Signal. 2006 Apr;18(4):499-507. Epub 2005 Jul 14.
Keywords
Anoxia/metabolismBiosensing Techniques/*methodsCell LineCell Membrane/metabolismEnzyme Inhibitors/pharmacologyHumansHypoxia-Inducible Factor 1, alpha Subunit/drug effects/metabolismNADPH Oxidase/antagonists & inhibitors/genetics/*physiologyNerve Tissue ProteinsOnium Compounds/pharmacologyOxygen/analysis/*metabolismPotassium/metabolism/physiologyPotassium Channels, Tandem Pore Domain/antagonists &inhibitors/*metabolismRNA, Small Interfering/genetics/pharmacology/physiologySignal Transduction/drug effects/physiology
Abstract
When oxygen sensing cells are excited by hypoxia, background K+ currents are inhibited. TASK-1, which is commonly expressed in oxygen sensing cells and makes a background K+ current, is inactivated by hypoxia. Thus TASK-1 is a candidate molecule responsible for hypoxic excitation. However, TASK-1 per se cannot sense oxygen and may require a regulatory protein that can. In the present study, we propose that the NADPH oxidase NOX4 functions as an oxygen-sensing partner and that it modulates the oxygen sensitivity of TASK-1. Confocal imaging revealed the co-localization of TASK-1 and NOX4 in the plasma membrane. In HEK293 cells expressing NOX4 endogenously, the activity of expressed TASK-1 was moderately inhibited by hypoxia, and this oxygen response was significantly augmented by NOX4. Moreover, the oxygen sensitivity of TASK-1 was abolished by NOX4 siRNA and NADPH oxidase inhibitors. These results suggest a novel function for NOX4 in the oxygen-dependent regulation of TASK-1 activity.
ISSN
0898-6568 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16019190

http://hdl.handle.net/10371/23437
DOI
https://doi.org/10.1016/j.cellsig.2005.05.025
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse