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Immunohistochemical study of p47Phox and gp91Phox distributions in rat brain

Cited 55 time in Web of Science Cited 63 time in Scopus
Authors
Kim, Myeung Ju; Shin, Kyung-Sue; Chung, Young-Bae; Jung, Kee Wook; Cha, Choong Ik; Shin, Dong Hoon
Issue Date
2005
Publisher
Elsevier
Citation
Brain Res. 1040 (2005) 178-186
Keywords
AnimalsBrain/metabolism*Brain Chemistry/physiologyImmunohistochemistryMaleMembrane Glycoproteins/*analysis/biosynthesisNADPH Oxidase/*analysis/biosynthesisPhosphoproteins/*analysis/biosynthesisRatsRats, Sprague-Dawley
Abstract
NADPH oxidase is multi-component enzyme, which comprises the cytosolic proteins p40Phox, p47Phox, and p67Phox and the two membrane proteins, gp91Phox and p22Phox, and which is well characterized in phagocytic cells. NADPH oxidase is a primary source of reactive oxygen species (ROS), and recent studies indicate that free radicals and ROS might be causative factors of several brain degenerative diseases and dysfunctions. However, though previous studies have shown the presence of NADPH oxidase subunits in cell culture and mouse brain, they have not provided detailed high power resolution data. Therefore, we investigated the distributions of the p47Phox and gp91Phox subunits in rat brain using immunohistochemical approach. Cortex, hippocampus, and Purkinje cells of cerebellum were prominently stained by p47Phox and gp91Phox antibodies. As compared with the distributions of p47Phox, gp91Phox in mouse, some differences in the rat brain were observed in the hippocampus, thalamus, amygdala, reticular nucleus, and basal ganglia. Additionally, at the cellular level, most p47Phox immunoreactivity was largely confined to cell bodies and proximal portions of the dendritic tree. Taken together, the widespread observed distributions of p47Phox and gp91Phox subunits indicate that they are probably needed to maintain normal brain function.
ISSN
0006-8993 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15804439

http://hdl.handle.net/10371/23538
DOI
https://doi.org/10.1016/j.brainres.2005.01.066
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College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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