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Membrane depolarization stimulates the proliferation of SH-SY5Y human neuroblastoma cells by increasing retinoblastoma protein (RB) phosphorylation through the activation of cyclin-dependent kinase 2 (Cdk2)

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Authors
Seo, Miran; Kim, Yeni; Lee, Yun-Il; Kim, So-Young; Ahn, Yong-Min; Kang, Ung Gu; Roh, Myoung-Sun; Kim, Yong-Sik; Juhnn, Yong-Sung
Issue Date
2006-07-11
Publisher
Elsevier
Citation
Neurosci Lett. 2006 Aug 14;404(1-2):87-92. Epub 2006 Jul 7.
Keywords
Cell Division/drug effects/*physiologyCell Line, TumorCell Membrane/drug effects/*physiologyCyclin E/metabolismCyclin-Dependent Kinase 2/*metabolismCyclin-Dependent Kinase 4/metabolismCyclin-Dependent Kinase 6/metabolismDNA, Neoplasm/biosynthesisEnzyme ActivationHumansMembrane PotentialsNeuroblastomaPhosphorylationPotassium Chloride/pharmacologyRetinoblastoma Protein/*metabolismThymidine/metabolism
Abstract
Membrane depolarization causes transmembrane ionic influxes that induce various gene expressions, and is involved in the processes of neuronal differentiation and apoptosis. However, the effect of membrane depolarization on neuronal proliferation has not been established. In this study, we aimed to investigate the effect of membrane depolarization on the proliferation of SH-SY5Y human neuroblastoma cells. Membrane depolarization induced by 50 mM KCl for 5 min significantly increased SH-SY5Y cell numbers and thymidine incorporation at 24 h after depolarization, and increased the phosphorylation and expression of retinoblastoma protein (RB), the activity of Cdk2 (without changing the activities of Cdk4 and Cdk6), and the expressions of cyclin A and cyclin E. Single and repeated depolarization (once a day for 6 days) had similar effects on RB, Cdks, and cyclins levels and activities. In summary, our results suggest that membrane depolarization may stimulate cellular proliferation by augmenting the expression of cyclin E leading to increases in Cdk2 activity and RB phosphorylation.
ISSN
0304-3940 (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T0G-4KBVX0Y-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=becc31f32ee66cb7c9d012914cfde19b

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16824683

http://hdl.handle.net/10371/23852
DOI
https://doi.org/10.1016/j.neulet.2006.05.061
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College of Medicine/School of Medicine (의과대학/대학원)Psychiatry (정신과학전공)Journal Papers (저널논문_정신과학전공)
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