Browse

Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration

Cited 101 time in Web of Science Cited 107 time in Scopus
Authors
Ki, Chang Seok; Park, Sook Young; Kim, Hyun Jeong; Jung, Hong-Moon; Woo, Kyung Mi; Lee, Jung Weon; Park, Young Hwan
Issue Date
2007-11-02
Publisher
Springer Verlag
Citation
Biotechnol Lett. 2008 Mar;30(3):405-10. Epub 2007 Nov 1.
Keywords
Biocompatible Materials*Bone RegenerationCell AdhesionCell LineCell ProliferationFibroins/*chemistryMicroscopy, Electron, Scanning*NanostructuresOsteoblasts/*cytology/physiology/ultrastructureOsteogenesisPorosityTissue Engineering/*methods*Tissue Scaffolds
Abstract
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.
ISSN
0141-5492 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17973083

http://hdl.handle.net/10371/23985
DOI
https://doi.org/10.1007/s10529-007-9581-5
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Program in Cancer Biology (협동과정-종양생물학전공)Journal Papers (저널논문_협동과정-종양생물학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse