S-Space College of Medicine/School of Medicine (의과대학/대학원) Program in Cancer Biology (협동과정-종양생물학전공) Journal Papers (저널논문_협동과정-종양생물학전공)
Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration
- Ki, Chang Seok; Park, Sook Young; Kim, Hyun Jeong; Jung, Hong-Moon; Woo, Kyung Mi; Lee, Jung Weon; Park, Young Hwan
- Issue Date
- Springer Verlag
- Biotechnol Lett. 2008 Mar;30(3):405-10. Epub 2007 Nov 1.
- Biocompatible Materials; *Bone Regeneration; Cell Adhesion; Cell Line; Cell Proliferation; Fibroins/*chemistry; Microscopy, Electron, Scanning; *Nanostructures; Osteoblasts/*cytology/physiology/ultrastructure; Osteogenesis; Porosity; Tissue Engineering/*methods; *Tissue Scaffolds
- We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.
- 0141-5492 (Print)
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