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Generation of reversible Rb-knockdown mice

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Authors
Choi, Yoon Sik; Lee, Jae Eun; Cheong, Cheolho; Sung, Young Hoon; Yang, Eun Young; Park, Chan Bae; Song, Jaewhan; Park, Sang Chul; Lee, Han-Woong
Issue Date
2005-08-10
Publisher
Elsevier
Citation
Mech Ageing Dev. 2005 Nov;126(11):1164-9.
Keywords
AnimalsCells, CulturedEmbryo, Mammalian/anatomy & histology/physiologyGene Expression Regulation, DevelopmentalMice*Mice, KnockoutMice, TransgenicPhenotypeRetinoblastoma Protein/genetics/*metabolism*Transgenes
Abstract
This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings P(hCMV)-tTA/TRE-Rb transgenic mice with conventional Rb+/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb-/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.
ISSN
0047-6374 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16087217

http://hdl.handle.net/10371/24321
DOI
https://doi.org/10.1016/j.mad.2005.06.002
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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