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8-Oxo-7,8-dihydro-2'-deoxyguanosine is not salvaged for DNA synthesis in human leukemic U937 cells

Cited 16 time in Web of Science Cited 17 time in Scopus
Authors
Kim, Ja-Eun; Chung, Myung-Hee
Issue Date
2006-03-23
Publisher
Taylor & Francis
Citation
Free Radic Res. 2006 May;40(5):461-6.
Keywords
Cell Line, TumorDNA/*metabolismDNA Replication/physiologyGuanine/*analogs & derivatives/metabolismHumansLeukemiaOxidative Stress/physiology
Abstract
8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG), the most common oxidatively modified nucleoside, is released from oxidized DNA and oxidized nucleotide pool. However, little information is available regarding the metabolic pathway of free 8-oxo-dG. In this study, we generated radiolabeled 8-oxo-dG to track its metabolic fate. We report that 8-oxo-dG is neither phosphorylated to 8-oxo-dGMP nor degraded to the free base, 8-oxo-7,8-dihydroguanine (8-oxo-Gua), indicating that 8-oxo-dG is not a substrate for nucleotide synthesis. This result was confirmed by the finding that no radioactivity was detected in the DNA of U937 cells after incubating the cells with radiolabeled 8-oxo-dG. These observations indicate that 8-oxo-dG produced by oxidative stress is not reutilized for DNA synthesis.
ISSN
1071-5762 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16551572

http://hdl.handle.net/10371/24403
DOI
https://doi.org/10.1080/10715760600570539
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College of Medicine/School of Medicine (의과대학/대학원)Pharmacology (약리학전공)Journal Papers (저널논문_약리학전공)
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