Browse

Regulation of insulin response in skeletal muscle cell by caveolin status

Cited 25 time in Web of Science Cited 26 time in Scopus
Authors
Oh, Yoon Sin; Cho, Kyung A.; Ryu, Sung Jin; Khil, Lee-Yong; Jun, Hee-Sook; Yoon, Ji-Won; Park, Sang Chul
Issue Date
2006-05-06
Publisher
Wiley-Blackwell
Citation
J Cell Biochem. 2006 Oct 15;99(3):747-58.
Keywords
AnimalsCaveolae/metabolism/ultrastructureCaveolin 1/genetics/*metabolismCaveolin 2/genetics/metabolismCaveolin 3/genetics/*metabolismCell Differentiation/physiologyCell LineGlucose/metabolismGlucose Transporter Type 4/genetics/metabolismInsulin/*metabolismMiceMuscle, Skeletal/cytology/*metabolismRNA, Small Interfering/genetics/metabolismRatsReceptor, Insulin/genetics/metabolismSignal Transduction/physiology
Abstract
Recent studies on the role of caveolin-1 in adipocytes showed that caveolin has emerged as an important regulatory element in insulin signaling but little is known on its role in skeletal muscle cells. In this study, we demonstrate for the first time that caveolin-1 plays a crucial role in insulin dependent glucose uptake in skeletal muscle cells. Differentiation of L6 skeletal muscle cells induce the expression of caveolin-1 and caveolin-3 with partial colocalization. However in contrast to adipocytes, phosphorylation of insulin receptor beta (IRbeta) and Akt/Erk was not affected by the respective downregulation of caveolin-1 or caveolin-3 in the muscle cells. Moreover, the phosphorylation of IRbeta was detected not only in the caveolae but also in the non-caveolae fractions of the muscle cells despite the interaction of IRbeta with caveolin-1 and caveolin-3. These data implicate the lack of relationship between caveolins and IRbeta pathway in the muscle cells, different from the adipocytes. However, glucose uptake was reduced specifically by downregulation of caveolin-1, but not that of caveolin-3. Taken together, these observations suggest that caveolin-1 plays a crucial role in glucose uptake in differentiated muscle cells and that the regulation of caveolin-1 expression may be an important mechanism for insulin sensitivity, implying the role of muscle cells for type 2 diabetes.
ISSN
0730-2312 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16676355

http://hdl.handle.net/10371/24729
DOI
https://doi.org/10.1002/jcb.20943
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse