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Role of Src-specific phosphorylation site on focal adhesion kinase for senescence-associated apoptosis resistance

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dc.contributor.authorRyu, S. J.-
dc.contributor.authorCho, K. A.-
dc.contributor.authorOh, Y. S.-
dc.contributor.authorPark, S. C.-
dc.date.accessioned2010-01-04T02:16:27Z-
dc.date.available2010-01-04T02:16:27Z-
dc.date.issued2006-03-09-
dc.identifier.citationApoptosis 2006; 11: 303-313en
dc.identifier.issn1360-8185 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16523241-
dc.identifier.urihttp://hdl.handle.net/10371/24808-
dc.description.abstractA decreased apoptotic response toward noxious stress is an issuing characteristic of the aging phenotype. Hydrogen peroxide or staurosporine induced apoptosis readily in young cells but not in senescent cells. We showed that focal adhesion kinase (FAK) expression and its phosphorylation at Tyr397, autophosphorylation site for focal adhesion formation, and Tyr577, Src-dependent phosphorylation site, were both increased in senescent cells. Moreover, FAK was inactivated proteolytically by apoptotic stimuli in young cells, but not in senescent cells. In addition, senescent cells whose FAK expression was downregulated by siRNA showed the increased level of apoptosis by staurosporine treatment via caspase-3 activation but not by hydrogen peroxide treatment. Interestingly dephosphorylation at Tyr577 of FAK by PP2 treatment, Src-family kinase inhibitor, induced the apoptosis by staurosporine in senescent cells but dephosphorylation at Tyr397 by downregulation of caveolin-1 was not affected. These data suggest that FAK might differently regulate apoptosis and focal adhesion formation through site-specific tyrosine phosphorylation in senescent cells.en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.subjectApoptosis/*physiologyen
dc.subjectCaspase 3/metabolismen
dc.subject*Cell Agingen
dc.subjectCell Cycleen
dc.subjectCells, Cultureden
dc.subjectCollagen Type XI/metabolismen
dc.subjectEnzyme Activationen
dc.subjectEnzyme Inhibitors/pharmacologyen
dc.subjectFibroblasts/cytology/drug effects/physiologyen
dc.subjectFocal Adhesion Protein-Tyrosine Kinases/genetics/*metabolismen
dc.subjectHumansen
dc.subjectHydrogen Peroxide/pharmacologyen
dc.subjectOxidants/pharmacologyen
dc.subjectPhosphorylationen
dc.subjectRNA, Small Interfering/metabolismen
dc.subjectStaurosporine/pharmacologyen
dc.subjectTyrosine/metabolismen
dc.subjectsrc-Family Kinases/antagonists & inhibitors/*metabolismen
dc.titleRole of Src-specific phosphorylation site on focal adhesion kinase for senescence-associated apoptosis resistanceen
dc.typeArticleen
dc.identifier.doi10.1007/s10495-006-3978-9-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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