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Altered renal sodium transporter expression in an animal model of type 2 diabetes mellitus

Cited 8 time in Web of Science Cited 9 time in Scopus
Authors
Oh, Yun Kyu; Joo, Kwon Wook; Lee, Jay Wook; Jeon, Un Sil; Lim, Chun Soo; Han, Jin Suk; Knepper, Mark A; Na, Ki Young
Issue Date
2007
Publisher
Korean Academy of Medical Science
Citation
J Korean Med Sci 2007; 22: 1034-41
Keywords
AnimalsBlood Glucose/analysisDiabetes Mellitus, Type 2/*metabolism*Disease Models, AnimalEpithelial Sodium Channel/*analysisHypertension/complicationsImmunoblottingImmunohistochemistryKidney/*metabolismMaleRatsSodium/*metabolismSodium-Hydrogen Antiporter/geneticsSodium-Potassium-Chloride Symporters/genetics
Abstract
Hemodynamic factors play an important role in the development and/or progression of diabetic nephropathy. We hypothesized that renal sodium transporter dysregulation might contribute to the hemodynamic alterations in diabetic nephropathy. Otsuka Long Evans Tokushima Fatty (OLETF) rats were used as an animal model for type 2 diabetes. Long Evans Tokushima (LETO) rats were used as controls. Renal sodium transporter regulation was investigated by semiquantitative immunoblotting and immunohistochemistry of the kidneys of 40-week-old animals. The mean serum glucose level in OLETF rats was increased to 235+/-25 mg/dL at 25 weeks, and the hyperglycemia continued up to the end of 40 weeks. Urine protein/ creatinine ratios were 10 times higher in OLETF rats than in LETO rats. At 40th week, the abundance of the epithelial sodium channel (ENaC) beta-subunit was increased in OLETF rats, but the abundance of the ENaC gamma-subunit was decreased. No significant differences were observed in the ENaC alpha-subunit or other major sodium transporters. Immunohistochemistry for the ENaC beta-subunit showed increased immunoreactivity in OLETF rats, whereas the ENaC gamma-subunit showed reduced immunoreactivity in these rats. In OLETF rats, ENaC beta-subunit upregulation and ENaC gamma-subunit downregulation after the development of diabetic nephropathy may reflect an abnormal sodium balance.
ISSN
1011-8934 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18162719

http://hdl.handle.net/10371/24894
DOI
https://doi.org/10.3346/jkms.2007.22.6.1034
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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