Publications
Detailed Information
Crystal structure of YrrB: a TPR protein with an unusual peptide-binding site
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Issue Date
- 2007-07-13
- Publisher
- Wiley-Blackwell
- Citation
- Biochem Biophys Res Commun. 2007 Sep 7;360(4):784-90. Epub 2007 Jul 5.
- Keywords
- Amino Acid Sequence ; Bacillus subtilis/chemistry ; Bacterial Proteins/*chemistry/metabolism ; Binding Sites ; Crystallography, X-Ray ; Molecular Sequence Data ; Peptides/*metabolism ; Protein Conformation ; Repetitive Sequences, Amino Acid ; Sequence Homology, Amino Acid
- Abstract
- YrrB is a hypothetical protein containing a tetratricopeptide repeat (TPR) domain from a Gram-positive bacterium, Bacillus subtilis. We determined YrrB structure in the C2 space group to 2.5A resolution, which is the first TPR structure of the Gram-positive bacterium B. subtilis. In contrast to other known TPR structures, the concave surface of the YrrB TPR domain is composed of the putative peptide-binding pocket lined with positively-charged residues. This unique charge distribution reveals that YrrB can interact with partner proteins via an unusual TPR-mediated interaction mode, compared to that of other TPR-containing structures. Functional annotation using genomics analysis suggested that YrrB may be an interacting mediator in the complex formation among RNA sulfuration components. No proteins containing a TPR domain have been identified in the biosynthesis of sulfur-containing biomolecules. Thus, YrrB could play a new role as a connecting module among those proteins in the conserved gene cluster for RNA sulfuration.
- ISSN
- 0006-291X (Print)
- Language
- English
- URI
- http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4P48623-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=842ac4e3b02bc01a6a714438ee40fa46
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17624311
https://hdl.handle.net/10371/27908
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.