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Noninvasive in vivo monitoring of neuronal differentiation using reporter driven by a neuronal promoter
DC Field | Value | Language |
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dc.contributor.author | Hwang, Do Won | - |
dc.contributor.author | Kang, Joo Hyun | - |
dc.contributor.author | Jeong, Jae Min | - |
dc.contributor.author | Chung, June-Key | - |
dc.contributor.author | Lee, Myung Chul | - |
dc.contributor.author | Kim, Soonhag | - |
dc.contributor.author | Lee, Dong Soo | - |
dc.date.accessioned | 2010-01-07T05:47:53Z | - |
dc.date.available | 2010-01-07T05:47:53Z | - |
dc.date.issued | 2007-09-22 | - |
dc.identifier.citation | Eur J Nucl Med Mol Imaging. 2008 Jan;35(1):135-45. Epub 2007 Sep 21. | en |
dc.identifier.issn | 1619-7089 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17885755 | - |
dc.identifier.uri | https://hdl.handle.net/10371/28346 | - |
dc.description.abstract | PURPOSE: We imaged neuronal differentiation in vivo using dual reporters (sodium iodide symporter [NIS] and luciferase) coupled to a neuron-specific enolase (NSE) promoter. METHODS: PC12 (NSE positive) and F11 cells were transfected with a bicistronic (NIS and luciferase; pNSE-NF) or a luciferase (pNSE-Fluc) reporter coupled to the NSE promoter. Weak NSE promoter activity was overcome by a two-step transcriptional amplification (TSTA) system (pNSE-TSTA-Fluc). In vivo, NIS and luciferase expression were examined using a (99m)Tc-pertechnetate gamma camera and bioluminescence imaging, respectively. RESULTS: pNSE-NF-transfected PC12 cells showed 3-fold higher radioiodine uptakes and >100-fold higher luciferase activity than parental cells. NIS or luciferase activity was not detected in pNSE-NF-transfected HeLa cells. When F11 cells were differentiated into neurons by db-cAMP, NIS and luciferase activities increased 4-fold compared to those without treatment, which was confirmed by Western blot and RT-PCR of NSE. In vivo in pNSE-NF-transfected F11 cells, db-cAMP treatment increased the luciferase activity but not the scintigraphic activity. In vitro, pNSE-TSTA-Fluc produced 130-fold higher luciferase activity than pNSE-Fluc and neuronal differentiation showed 4-fold higher activity from both pNSE-TSTA-Fluc and pNSE-Fluc than before differentiation. In vivo, in pNSE-TSTA-Fluc-transfected F11 cells, luciferase activity increased after neuronal differentiation. In vivo luciferase activity persisted up to 2 days after db-cAMP-induced neuronal differentiation. CONCLUSION: NSE promoter-driven dual reporter transgenes revealed the possibility of in vivo imaging of neuronal differentiation, which was further enabled by high amplification using a TSTA system. We propose that this strategy be used to follow the transplanted stem cells during differentiation in live animals. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Animals | en |
dc.subject | Cell Differentiation/*genetics | en |
dc.subject | Cell Line | en |
dc.subject | Gene Expression Regulation | en |
dc.subject | Genes, Reporter/*genetics | en |
dc.subject | Genetic Vectors | en |
dc.subject | Humans | en |
dc.subject | Iodine Radioisotopes | en |
dc.subject | Luciferases/genetics/metabolism | en |
dc.subject | Luminescence | en |
dc.subject | Neurons/*cytology/*metabolism/radionuclide imaging | en |
dc.subject | Phosphopyruvate Hydratase/genetics/metabolism | en |
dc.subject | Promoter Regions, Genetic/*genetics | en |
dc.subject | Rats | en |
dc.subject | Symporters/genetics/metabolism | en |
dc.subject | Transfection | en |
dc.title | Noninvasive in vivo monitoring of neuronal differentiation using reporter driven by a neuronal promoter | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 황도원 | - |
dc.contributor.AlternativeAuthor | 강주현 | - |
dc.contributor.AlternativeAuthor | 정재민 | - |
dc.contributor.AlternativeAuthor | 정준기 | - |
dc.contributor.AlternativeAuthor | 이명철 | - |
dc.contributor.AlternativeAuthor | 김순학 | - |
dc.contributor.AlternativeAuthor | 이동수 | - |
dc.identifier.doi | 10.1007/s00259-007-0561-8 | - |
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