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Visualization of endogenous p53-mediated transcription in vivo using sodium iodide symporter

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dc.contributor.authorKim, Kwang Il-
dc.contributor.authorChung, June-Key-
dc.contributor.authorKang, Joo Hyun-
dc.contributor.authorLee, Yong Jin-
dc.contributor.authorShin, Jae Hoon-
dc.contributor.authorOh, Hyun Jeong-
dc.contributor.authorJeong, Jae Min-
dc.contributor.authorLee, Dong Soo-
dc.contributor.authorLee, Myung Chul-
dc.date.accessioned2010-01-07T05:53:47Z-
dc.date.available2010-01-07T05:53:47Z-
dc.date.issued2005-01-27-
dc.identifier.citationClin Cancer Res. 2005 Jan 1;11(1):123-8.en
dc.identifier.issn1078-0432 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15671536-
dc.identifier.urihttp://hdl.handle.net/10371/28352-
dc.description.abstractPURPOSE: To develop a gamma camera imaging method for the determination of endogenous gene expression, we evaluated the expression of endogenous p53 gene using human sodium iodide symporter (hNIS) gene as reporter. EXPERIMENTAL DESIGN: We constructed cis-p53RE-hNIS reporter vector placed under control of an artificial enhancer (p53RE). Moreover, we transfected it into human hepatoma cell line SK-Hep1 by liposome. Geneticin was used for the selection of stable transfectant (SK-Hep1p53NIS). To evaluate the function of hNIS, the inhibition study was examined with 1 mmol/L potassium perchlorate. After treatment of Adriamycin with serial dose for 24 hours, we measured the uptake of 125I and did Western blot analysis to evaluate expression of p53 protein. Tumor xenografts were produced in nude mice by s.c. injection of SK-Hep1p53NIS cells. After 7 days, scintigraphic images of nude mice before and after Adriamycin treatment were obtained using [99mTc]-pertechnetate. RESULTS: In the SK-Hep1p53NIS cells, Adriamycin-treated cells accumulated up to three times higher than did nontreated cells. Potassium perchlorate inhibited completely the uptake of 125I. As Adriamycin dose increased, radioiodide uptake was significantly correlated with activated p53 as well as total p53 protein level. When Adriamycin (2 mg/kg) was treated in the same mice, a significantly higher uptake of [99mTc]-pertechnetate was observed in SK-Hep1p53NIS xenografts compared with nontreated xenografts (P < 0.05, unpaired t test). CONCLUSIONS: These results suggest that p53 expression level can be monitored by NIS gene expression using cis-p53RE-hNIS system in vitro and in vivo.en
dc.language.isoen-
dc.publisherAmerican Association for Cancer Researchen
dc.subjectAnimalsen
dc.subjectBiological Transporten
dc.subjectBlotting, Westernen
dc.subjectCell Line, Tumoren
dc.subjectDNA, Complementary/metabolismen
dc.subjectDose-Response Relationship, Drugen
dc.subjectDoxorubicin/pharmacologyen
dc.subject*Gene Expression Regulation, Neoplasticen
dc.subjectGenes, Reporteren
dc.subjectGenetic Vectorsen
dc.subjectGentamicins/pharmacologyen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Inbred BALB Cen
dc.subjectMice, Nudeen
dc.subjectNeoplasm Transplantationen
dc.subjectPerchloric Acid/pharmacologyen
dc.subjectPlasmids/metabolismen
dc.subjectPotassium Compounds/pharmacologyen
dc.subjectRadionuclide Imagingen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectSymporters/*metabolismen
dc.subjectTime Factorsen
dc.subject*Transcription, Geneticen
dc.subjectTranscriptional Activationen
dc.subjectTransfectionen
dc.subjectTumor Suppressor Protein p53/*metabolismen
dc.subjectUp-Regulationen
dc.titleVisualization of endogenous p53-mediated transcription in vivo using sodium iodide symporteren
dc.typeArticleen
dc.contributor.AlternativeAuthor김광일-
dc.contributor.AlternativeAuthor정준기-
dc.contributor.AlternativeAuthor강주현-
dc.contributor.AlternativeAuthor이용진-
dc.contributor.AlternativeAuthor신재훈-
dc.contributor.AlternativeAuthor오현정-
dc.contributor.AlternativeAuthor정재민-
dc.contributor.AlternativeAuthor이동수-
dc.contributor.AlternativeAuthor이명철-
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College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
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