Browse

Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAF600E mutation-prevalent area

Cited 146 time in Web of Science Cited 152 time in Scopus
Authors
Chung, Ki-wook; Yang, Sun Kyung; Lee, Geon Kook; Kim, Eun Young; Kwon, Soim; Lee, Sang Hyun; Park, Do Joon; Lee, Hyun Sook; Cho, Bo Youn; Lee, Eun Sook; Kim, Sun Wook
Issue Date
2006-10-24
Publisher
Wiley-Blackwell
Citation
Clin Endocrinol (Oxf). 2006 Nov;65(5):660-6.
Keywords
Adenocarcinoma, Follicular/geneticsAdultAgedBiopsy, Fine-NeedleCarcinoma, Papillary/ethnology/*genetics/pathologyCarcinoma, Papillary, Follicular/geneticsDNA Mutational AnalysisDNA, Neoplasm/analysisDiagnosis, DifferentialFemaleHumansKoreaMaleMiddle Aged*Point MutationPolymorphism, Restriction Fragment LengthProspective StudiesProto-Oncogene Proteins B-raf/*geneticsSensitivity and SpecificityThyroid Neoplasms/ethnology/*genetics/pathologyThyroid Nodule/ethnology/*genetics/pathology
Abstract
BACKGROUND: Between 10 and 30% of the fine needle aspiration biopsies (FNABs) of thyroid nodules are diagnosed as 'indeterminate'. A molecular diagnostic method is needed to reduce unnecessary surgery in this group. In Korea, most thyroid cancer is the classic papillary type and the BRAF(V600E) mutation is highly prevalent. AIM: To evaluate the role of pre-operative detection of BRAF(V600E) mutation in the FNAB specimens of thyroid nodules in a BRAF(V600E) mutation-prevalent geographical area. PATIENTS AND METHODS: In 137 specimens of FNAB (107 papillary thyroid carcinomas (PTC); 3 follicular thyroid carcinomas (FTC); 2 undifferentiated thyroid carcinomas; 25 benign lesions), both direct DNA sequencing and PCR-RFLP were used for detecting the BRAF(V600E) mutation. The sensitivity and specificity were calculated. We analysed the association between BRAF(V600E) mutation and the clinico-pathological parameters. RESULTS: The BRAF(V600E) mutation was present in 93 (83%) of 112 thyroid cancers. Direct DNA sequencing showed a sensitivity of 83.0% and a specificity of 96.0%. The sensitivity and specificity of PCR-RFLP were 78.6% and 80.0%, respectively. Among 25 cases with indeterminate FNAB cytology, 8 patients had malignant lesions (5 PTC and 3 FTC). Three (60%) of 5 PTCs and 1 out of 17 benign lesions had BRAF(V600E) mutation (only one false positive case and the definitive pathology showed atypical nodular hyperplasia that could be a premalignant lesion). The diagnostic accuracy of this molecular method in only the 25 indeterminate nodules was 76% (19/25). No mutation was found in 3 FTCs. Among 107 PTCs, there was no significant association of the BRAF(V600E) mutation with the known risk factors. CONCLUSION: Detection of the BRAF(V600E) mutation in FNAB specimens refines the FNAB-cytology diagnosis, especially in a BRAF(V600E) mutation-prevalent area. Direct DNA sequencing was a more reliable method than PCR-RFLP for detecting the BRAF(V600E) mutation with a high sensitivity and specificity.
ISSN
0300-0664 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17054470

http://hdl.handle.net/10371/28469
DOI
https://doi.org/10.1111/j.1365-2265.2006.02646.x
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse